Attenuated recombinant newcastle disease virus and vaccine containing the same

ABSTRACT

The present invention relates to a recombinant vector for transcription of the Newcastle disease virus (NDV) genome, a strain of attenuated recombinant NDV with a surface antigen of pathogenic NDV prepared by the vector, a method of preparing a recombinant NDV having low pathogenicity and high protectivity efficiency against Newcastle disease (ND) using the vector, and a vaccine against ND containing the recombinant NDV.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to and the benefit of Korean Patent Application No. 10-2006-0093260 filed in the Korean Intellectual Property Office on Sep. 26, 2006, the entire content of which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

(a) Field of the Invention

The present invention relates to a recombinant vector for transcription of the Newcastle disease virus (NDV) genome, a strain of attenuated recombinant NDV with a surface antigen of pathogenic NDV prepared by the vector, a method of preparing a recombinant NDV having low pathogenicity and high protective efficiency against Newcastle disease (ND) using the vector, and a vaccine against ND containing the recombinant NDV.

(b) Description of the Related Art

Newcastle Disease (ND), which is known as one of the most important internationally known kinds of livestock disease, is an acute thermal respiratory disease, and is the first communicable disease by law in Korea. If an unimmunized fowl is infected, the mortality is 100%. Because the Newcastle disease virus (NDV) commonly exists in Korea, many difficulties are expected for eradicating the disease. Also, because various Newcastle disease viruses are widespread in Southeast Asia, China, and Taiwan, all of which actively trade with Korea, and because these viruses are potentially very dangerous factor, an urgent need to develop an Asia-type Newcastle disease vaccine exists.

The Newcastle disease virus (NDV) is a single-stranded RNA virus belonging to the Avulavirus genus. The envelope of the Newcastle disease virus includes the Haemagglutinin-Neuraminidase (HN) protein which makes the virus bind to a host, and the Fusion (F) protein which makes the envelope fuse with the host cell. F and HN proteins are glycoproteins and are distributed on the surface of the viral envelope.

The F protein belongs to the type I membrane glycoprotein group and forms a trimeric structure (trimer). The F protein is made as a non-active precursor form (F0), and is divided into the disulfide linked subunits F1 and F2 when the precursor F0 molecule passes through Golgi membranes. This process exposes a hydrophobic domain at the amino terminus of the F1 subunit, wherein the domain performs important role in the biological activity of mature protein. The hydrophobic domain, called a fusion peptide, is well conserved in the F protein of paramyxovirus, and is directly involved in membrane fusion. The F protein of paramyxovirus includes common structure characteristics such as heptad repeats, and two regions being capable of forming an alpha helix structure. The longest heptad repeat A of the repeats is adjacent to the N-terminal hydrophobic fusion peptide of F1, and heptad repeat B is close to the upper part of transmembrane region. Heptad repeat B consists of a series of well-conserved Leucine or Isoleusine at every 7 residues.

HN protein belongs to the type II membrane glycoprotein and forms a tetramer on the surface of the viral envelope, to penetrate into a cell membrane (Gorman et al., 1988; Ng et al., 1989). The HN protein causes virion to locate on the host cell surface via binding to sialic acids of glycoconjugates. The HN protein is divided into the three regions of a transmembrane domain, a stalk domain and a globular domain. Both a binding site of an antigenic receptor and an active site of neuraminidase locate on the globular domain. An active site of fusion induction locates on the stalk domain, and interacts with the F protein (Sergei et al., 1993). The expected structure of the stalk domain is an alpha-helix structure, with two heptad repeats including the heptad repeat A (at the 74^(th)-88^(th) positions) and the heptad repeat B (at the 96^(th)-110^(th) position). Also, it has been reported that any mutation breaking the structure reduces the receptor binding and the neuraminidase activity. Moreover, it has been reported that a mutation capable of destroying a structure causes decrease of the receptor binding and neuraminidase activities.

According to the level of disease in chicken, NDV is classified with the following pathogenic types (pathotypes): 1) viscerotropic velogenic (high-pathogenic) NDV showing digestive organ lesions and high mortality; and neurotropic velogenic NDV mainly showing respiratory and neurological symptom, and high mortality; 2) mesogenic NDV showing low mortality, but acute respiratory and neurological symptoms in some of the poultry; 3) lentogenic (low-pathogenic) and apathogenic NDV causing slight illness or asymptomatic respiratory infection.

In order for the NDV to infect a cell, it is necessary for the precursor glycoprotein F0 to be cleaved into F1 and F2. This post-translational cleavage is intervened by proteases of a host cell. If the cleavage does not occur, non-infectious virions are generated, and the virus replication cannot progress. The F0 protein of a virulent virus can be cleaved by various proteases, but the F0 protein of a low toxicity virus is restricted in terms of susceptibilities, and particularly the low toxicity virus is only capable of growing in a specific host cell type.

Whereas the lentogenic virus is only reproduced in a region that has trypsin-like enzymes, including respiratory organs or intestinal tract, because the virulent viruses are reproduced in various regions including tissues and organs, and therefore the virulent viruses cause a fetal systemic infection.

By the amino acid inspection of the Fo precursor, it is identified that the lentogenic viruses have a single arginine (R) connecting F2 and F1 subunit, whereas strains with toxicity of more than the mesogenic have additional basic amino acids forming two pairs such as K/R-X-K/R-R-F on the cleavage region. Moreover, the F2 chain of the strains with pathogenicity of more than the mesogenic is generally is disclosed by Phenylalanine residue, whereas the F2 chain of the strains with pathogenicity of less than the lentogenic is generally disclosed by Leucine.

In the U.S., a killed vaccine has been used for the identification of the Newcastle disease (Hofstad, 1953). By observation that a part of the enzootic disease virus only generates mild disease, for the first time, the mesogenic live vaccine Roakin was developed, and subsequently, milder Hitchner B1 and LaSota (Goldhaft, 1980) was developed.

One of the main advantages of live vaccines is the capability of administering by using a mass application method of a low-cost. A conventional application method is to administer the vaccine through drinking water.

The mass application of a live vaccine through spray and aerosols is very useful, because many birds can be more quickly administered with the vaccine and thereby vaccinated. It is important to control nozzles wherein particles are generated, to achieve an exacting particle size.

Recently used live vaccines have some problems. Because these vaccines still have a little pathogenicity, occasionally side effects of the vaccine can occur. Moreover, because antibodies inherited from the maternal line neutralize live vaccine viruses, successful immunity formation can be interfered with. Therefore, it is important to use an exceedingly mild virus, to perform the first vaccination, and a vaccine that is able to overcome the maternal antibody is required.

A killed vaccine is usually produced from infectious allantoic fluid mixed with appropriate supplements, and it is treated with formalin or beta-propiolactone, to kill the virus. The vaccine is administered to muscles or through subcutaneous injection, but it has a disadvantage due to a high-cost for its production and application.

Recently, the antigenicity of velogenic NDV generated both domestically and in foreign countries has been presumed to show many differences compared with the vaccine strain. For this reason, it can be concluded that the discovery of an outdoor strain with many differences with a genotype of the vaccine strain may occur, and if the vaccine antibody titer is not high enough, it is able to prevent mortality, but it is unable to prevent from decreasing in egg-laying rate, etc.

Depending on a phylogenic analysis based on the partial sequence of the F gene, the genotype of NDV is classified as genotype I to genotype IX. Molecular epidemiologically, most Newcastle disease viruses distributed in Korea belong to genotype VI and genotype VII. In the case of genotype VI, even though a variant strain may occur because of intensive vaccination, relatively lowly isolated than the genotype VII, and primarily only the genotype VII has been isolated after 2000, the possibility of its extermination is considered. Consequently, sequencing analysis, a determination of recent NDV gene sequences through genome project, and a molecular epidemiological research through gene comparison with world-wide NDV that is registered in the GenBank are very important for developing an optimal vaccine strain.

At present, conventionally used killed oil vaccine of Newcastle disease (ND) is produced by using lentogenic NDV such as Clone 30 or a LaSota strain, and the production of a killed vaccine using velogenic NDV is prohibited because of safety problems. Therefore, the necessity for technology for producing an ND vaccine that is safer, more economical, and in which the antigen is similar to the field strain is increased, and the development of a vaccine using reverse genetics technology is mostly closed technology to this demand.

The reverse genetics technology of a negative strand RNA virus is proposed as a technology for a rescue of infectious virus from the virus genome (U.S. Pat. No. 5,166,057). Even though this technology was originally proposed to manipulate the influenza virus genome, it can be successfully applied to various segment and non-segment negative strand RNA viruses, including Rabies virus, Respiratory Syncytial virus, and Sendai virus.

The present inventors developed a novel vaccine strain using the reverse genetics technology as described above, and as a result; the technology of producing safe ND vaccine strain with antigenicity similar to that of the field strain is developed.

SUMMARY OF THE INVENTION

An object of the present invention is to provide a recombinant vector for transcription of the NDV genome.

Another object of the present invention is to provide a strain of attenuated recombinant NDV with a surface antigen of pathogenic NDV.

Another object of the present invention is to provide a method of preparing a recombinant NDV having low pathogenicity and high protective efficiency against Newcastle disease (ND) using the vector.

Another object of the present invention is to provide a method of attenuating NDV to be able to increase immunogenicity and decrease pathogenicity of NDV using reverse genetics technology.

Still another object of the present invention is to provide a vaccine against ND containing the recombinant NDV.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows a RT-PCT result, and the name and the location of the amplified RT-PCR products using genome RNA of the domestic velogenic Newcastle disease virus, KBNP-4152.

FIG. 2 shows a result of cloning the amplified product of FIG. 1 into a TA-cloning vector.

FIG. 3 shows a result of cloning the amplified product of FIG. 1 into a TA-cloning vector.

FIG. 4 shows a linker sequence used for manufacturing the parental vector, pTMH that is used for cloning the genomic DNA of NDV and a primer sequence used for manufacturing the linker.

FIG. 5 shows a schematic diagram of a process of manufacturing the pTMH vector.

FIG. 6 shows nucleotide sequences of the main site in the pTMH vector.

FIG. 7 shows the whole nucleotide sequence of the pTMH vector.

FIG. 8 shows a RT-PCT result, and the name and the location of the amplified RT-PCR products using genomic RNA of the La Sota strain.

FIGS. 9A and 9B show a result of cloning the amplified product of FIG. 8 into a TA-cloning vector.

FIGS. 10A and 10B show a process of cloning the genomic DNA of NDV into a pBR322 vector.

FIG. 11 shows a process of manufacturing plasmid for expressing the NP, P and L genes of NDV, wherein lanes 2, 3 and 4 of A indicate a RT-PCR result of NP, P and L genes, respectively, lanes 6, 7 and 8 of A indicate a gene inserted in a vector that is treated with NotI after cloning the NP, P and L genes into a pcDNA3.1/TOPO vector, respectively, and B indicates a schematic diagram outlining this.

FIG. 12 shows a semi-synthesis process of the F and HN genes using s PTDS technique.

FIG. 13 shows a nucleotide sequence of the furin-like enzyme recognition site of the F protein and the linker of M and F genes of the recombinant virus.

FIG. 14 shows a synthesis process of a gene wherein a mutation is caused in the furin-like enzyme recognition site of the F protein using PTDS and site-directed mutagenesis.

FIG. 15 shows a design drawing of PTDS primer for connecting HN (1-566) gene of KBNP-4152 with HN terminus (567-577) gene of La Sota strain.

FIG. 16 shows a process of manufacturing the recombinant virus, KBNP-C4152R2L.

FIG. 17 shows a process of manufacturing a clone of a recombinant virus with various furin-like enzyme recognition sites.

FIG. 18 is a result of showing whether KBNP-C4152R2L inoculated into a chicken embryo is increased or not by using a plate hemagglutination test.

FIG. 19 shows a pathotype-specific RT-PCR result for confirming the pathogenicity of KBNP-C4152R2L.

FIG. 20 is a result of comparing a nucleotide sequence of the furin-like enzyme recognition site of the F protein of KBNP-4152 and KBNP-C4152R2L.

FIG. 21 shows an antigenic relationship among the La Sota strain, KBNP-4152 and KBNP-C4152R2L by using a cross hemagglutination inhibition test.

FIG. 22 shows a cytophatic effect of KBNP-C4152R2L.

FIG. 23 shows a map of the KBNP-C4251R2L genome cloned into a pTMH vector and a nucleotide sequence of the genome.

FIG. 24 shows an F gene of the present invention and a phylogenic analysis result of Newcastle virus using neighbor-joining method.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention relates to a recombinant vector for transcription of a Newcastle disease virus (NDV) genome, a strain of attenuated recombinant NDV with a surface antigen of pathogenic Newcastle disease virus prepared by the vector, a method of preparing a recombinant NDV having low pathogenicity and high preventive efficiency against Newcastle disease (ND) using the vector, and a vaccine against Newcastle disease containing the recombinant Newcastle disease virus.

An F gene (384 bp; positioned at the 1^(st) to 384^(th) nucleotides in the F gene) of the present invention and a phylogenic analysis result of Newcastle virus using neighbor-joining method is shown in FIG. 24.

Strains shown in FIG. 24 are only examples, and currently numerous kinds of Newcastle disease virus strains are classified with genotype I to IX. Further, it has been much reported that the Newcastle virus is classified by molecular epidemiological analysis. Therefore, the classification of strains each genotype as described in FIG. 24 can be readily recognized by persons skilled in the art of the technical field to which this invention belongs. In this specification, the standard of the strain classification thereof quote a content written in reference as follows, the entire content of which is incorporated herein by reference:

-   1. Kwon H. J. PhD Thesis. Seoul National University, 2000. -   2. Lomniczi B., Wehmann E., Herczeg J., Ballagi-Pord     ny A., Kaleta E. F., Werner O., Meulemans G., Jorgensen P. H.,     Mante A. P., Gielkens A. L. J., Capua I., and Damoser J., Arch Virol     143, 49-64, 1998. -   3. Herczeg J., Wehmann E., Bragg R. R., Travassos Dias P. M.,     Hadjiev G., Werner O., and Lomniczi, B. Arch Virol 144, 2087-2099,     1999. -   4. Yang C. Y., Shieh H. K., Lin Y. L., Chang P. C., Avian Dis 43,     125-130, 1999. -   5. Kwon H. J., Cho S. H., Ahn Y. J., Seo S. H., Choi K. S., and     Kim S. J. Vet Microbiol 95, 39-48, 2003. -   6. Liu X. F., Wan H. Q., Ni X. X., Wu Y. T., and Liu W. B. (2003).     Pathotypical and genotypical characterization of strains of     Newcastle disease virus isolated from outbreaks in chicken and goose     flocks in some regions of China during 1985-2001. Arch Virol, 148,     1387-1403. -   7. Tsai H. J., Chang K. H., Tseng C. H., Frost K. M., Manvell R. J.,     and Alexander D. J. Vet Microbiol, 104, 19-30, 2004.

At present, La Sota/46 used as vaccine strain is genotype II, whereas most of strains identified as field strains belong to genotypes VI to VII, which are genetically distant from the vaccine strain La Sota/46. For example, in the HN protein of NDV, the 345-PDEQDYQIR-353 site on the HN protein has been known as an important linear epitope forming a neutralizing antibody. As domestic pathogenic NDV, genotype VI (95-98, 99-70, 99-71) of the virus and genotype VII of the virus have coexisted, and particularly, the genotype VI of NDV was isolated in 1999, but was not isolated from 2000 to 2006 at all. For the first time, genotype VIIa of NDV was isolated from fowls in 1995, and thereafter the virus was not isolated, and only genotype VIId of NDV isolated. In the case of genotype VI of virus, the first variant strain of linear epitope (E347K) was observed in strains (SNU9358GG, SNU9444) isolated in 1993 and 1994, and continuously observed in the 95-98, 99-70, and 99-71. Therefore, it is considered that these variant strains could avoid immunity and survive a long time, and is considered that the variant strains nearly are replaced with emerging genotype VII in Korea since 2000. In the case of genotype VII of the virus, all viruses isolated from 1995 to 2001 are similar to the linear epitope of the La Sota strain. However, variant strain of the linear epitope (E347K) was firstly observed in 2002, and predominantly NDV having additional mutation was observed in 2005 (refer to below Table).

Classical epitope Variant epitope Year 346DEQDYQIRM354 346-K ------ M/K-354 1995 1 — 2000 16 — 2001 1 — 2002 15 (79%) 4 (10.5%) 2003 1 1 2004 — 1 2005   1 (6.25%) 15 (93.75%) 2006 — 2

Considering this point, because the previous vaccine strain, La Sota/46 cannot used for effective prevention against antigenically different Newcastle disease virus emerging in current, therefore, it is very significant that the present invention provides the technology for developing a vaccine strain having almost similar antigenicity against field strain.

As used herein, the term “high-pathogenic (velogenic) Newcastle disease virus” includes pathogenic Newcastle disease viruses having pathogenicity equal to or higher than that of the mesogenic strain as well as conventionally classified high-pathogenic Newcastle disease viruses, unless it is differently defined. In the present invention, the high-pathogenic Newcastle disease virus shows its pathogenicity by producing infectious viruses in all cells of the body when infected into an animal. When the case of an amino acid sequence at the 113^(th) to 116^(th) positions of the F protein is represented by following Formula 1, the F protein is cleaved by furin or furin-like protease (hereinafter referred to as ‘furin’) existing in almost all cells in the body, thereby being converted into an active structure, and obtaining infectious ability. Therefore, a pathogenic Newcastle disease virus is defined as one having nucleotide sequence encoding an amino acid sequence represented by following Formula 1 at the 113^(th) to 116^(th) positions of the F protein: 113-X₁X₂X₃X₄-116  Formula 1

wherein

X₁, X₃ and X₄ are independently arginine (R) or lysine (K), and

X₂ is selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophan, valine, asparagine, cysteine, glutamine, glycine, serine, theronine, tyrosine, aspartic acid, glutamic acid, arginine, histidine, and lysine.

In this case, when an amino acid at the 112^(th) position of the F protein is a basic amino acid such as arginine or lysine, the pathogenic Newcastle disease virus has higher pathogenicity.

Also, as used herein, the term “low-pathogenic (lentogenic) Newcastle disease virus” includes apathogenic Newcastle disease viruses having no pathogenicity as well as conventionally classified lentogenic Newcastle disease viruses, unless differently defined. In the present invention, the low-pathogenic Newcastle disease virus having nucleotide sequence encoding an amino acid sequence represented by following Formula 2 at the 113^(th) to 116^(th) positions of the F protein, and when infected into animals, the virus is activated only by some specific extracellular protease in digestive organs and respiratory organs, thereby being merely locally infected to show low pathogenicity. Therefore, the low-pathogenic Newcastle disease virus is defined as having nucleotide sequence encoding an amino acid sequence represented by following Formula 2 at the 113^(th) to 116^(th) positions of the F protein: 113-X₄X₅X₇X₈-116  Formula 2

wherein

X₅, X₆ and X₇ are independently selected from the group consisting of alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophan, valine, asparagine, cysteine, glutamine, glycine, serine, theronine, tyrosine, aspartic acid, glutamic acid, arginine, histidine and lysine, and X₅ and X₇ are not both arginine (R) or lysine (K) at the same time, and

X₈ is arginine (R) or lysine (K).

The Newcastle disease virus obtains pathogenicity when a cleavage site positioned in F protein of the virus is cleaved by furin and a fusion region where the F protein is fused with a cellular membrane is exposed. furin is an enzyme distributed in the whole body of an animal, and thus, the activation of the infectious ability of the virus by furin can be generated in the whole body of animal, whereby the virus has pathogenicity. Therefore, the level of pathogenicity of the Newcastle disease virus may depend on the extent of recognition and cleavage by furin at the cleaved site in F protein.

When the furin recognition site (amino acids at 113^(th) to 116^(th) positions) of F protein of the Newcastle disease virus has at least three basic amino acids (113-R-X-K/R-R-116) as shown in Formula 1, the virus can be systemically infected, and obtain pathogenicity. However, as shown in Formula 2, if one or more basic amino acids are replaced with a non-basic amino acid, the cleavage and recognition are conducted by not furin but locally existing extracellular protease, and mortal systemic infection of the virus does not occur, thereby showing low pathogenicity.

The present invention relates to a technique to prepare a genetically stable and attenuated recombinant Newcastle disease virus based on a low-pathogenic Newcastle disease virus, by substituting coding regions for the F protein and HN protein with those of high-pathogenic viruses prevailing domestically and in Asia, to enhance the protective effect of the virus against the high-pathogenic viruses, and by substituting a codon encoding the 115^(th) amino acid of a high-pathogenic Newcastle disease virus with a codon for a non-basic amino acid, wherein the codon for a non-basic amino acid can be converted into a codon for a basic amino acid when at least two point mutations occur. As described above, the recombinant Newcastle disease virus according to the present invention has an identical or similar surface antigen with field strains thereby showing high antigenicity against the field strains and being effectively attenuated. In addition, the recombinant Newcastle disease virus according to the present invention cannot be converted into a high-pathogenic strain until at least two point mutations occur at the codon for the 115^(th) amino acid of the F protein, thereby showing excellent stability and safety.

Pathogenic Newcastle disease viruses can be classified into a syncytial type forming syncytia and a granular type forming granules depending on their cytopathic effects, and it is generally known that the syncytial type is more highly pathogenic than the granular type. The present invention may be characterized by remarkably decreasing the pathogenicity by using the granular type virus clone as a high-velogenic Newcastle disease virus for providing the region encoding the F and HN proteins. In addition, in an HN protein of the Newcastle disease virus, the HN protein of a high-pathogenic Newcastle disease virus has 571 amino acids, which is a relatively short length, whereas the HN protein of a low-pathogenic Newcastle disease virus has 577 or 616 amino acids, which is longer than that of the high-pathogenic virus, and thus the high-pathogenic and the low-pathogenic strains may be classified by a C-terminal amino acid sequence of HN. Therefore, in the present invention, the C-terminus of HN protein is modified identically with that of a low-pathogenic strain (577 amino acids), thereby preparing a more attenuated recombinant Newcastle disease virus.

As used herein, the nucleotide sequences encoding P, M, F, HN and L proteins contained in a vector for transcription of the Newcastle disease virus genome and a recombinant Newcastle disease virus should be understood to include all non-coding nucleotide sequences existing in P, M, F, HN and L genes as long as they have no affectations on the expressed proteins, as well as the nucleotide sequences directly encoding the proteins.

In more detail, the present invention relates to a vector for transcription of the Newcastle disease virus genome, including a gene fragment consisting of nucleotide sequences encoding NP, P, M, F, HN, and L proteins of the Newcastle disease virus; and a promoter and a terminator, that are operably linked to the gene fragment,

wherein the NP, P, M, and L genes are derived from the genome of a low-pathogenic Newcastle disease virus, the La Sota strain, and the F and HN genes are derived from the genome of a high-pathogenic Newcastle disease virus, KBNP-4152,

wherein the F protein coding sequence contained in the vector is characterized in that the codon encoding basic amino acid at the 115^(th) position of the F protein of the velogenic Newcastle disease virus including KBNP-4152 is substituted with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU.

The HN gene of the vector may be additionally mutated so that the codons for the 1^(st) to 569^(th) amino acids of HN protein encodes those of a high-pathogenic Newcastle disease virus and the codons for the amino acids after 570^(th) position encode those of a low-pathogenic Newcastle disease virus including the La Sota strain.

In addition, the promoter and terminator may be used without particular limits, as long as they can be operably linked to the Newcastle disease virus genome in the plasmid. Such promoters and terminators may be readily selected and used by persons skilled in the art of the technical field to which this invention belongs. In an embodiment of the present invention, the promoter and terminator may be a T7 promoter and a T7 terminator, respectively.

In the preferred embodiment of the present invention, the vector for transcription of the virus genome may include nucleotide sequences encoding amino acid sequences of SEQ ID NO: 2 to 7 or nucleotide sequence of following SEQ ID NO: 1 (refer to FIG. 23, hereinafter, called pTNH-c4152R2L).

                                                       Gene start 1 accaaacagagaatccgtgagttacgataaaaggcgaaggagcaattgaagtcgc acggg 61 tagaa ggtgtgaatctcgagtgcgagcccgaagcacaaactcgagaaagccttctgccaa   M  S  S  V  F  D  E  Y  E  Q  L  L  A  A  Q  T  R  P  N  G• 121 cATGTCTTCCGTATTTGATGAGTACGAACAGCTCCTCGCGGCTCAGACTCGCCCCAATGG  •A  H  G  G  G  E  K  G  S  T  L  K  V  D  V  P  V  F  T  L• 181 AGCTCATGGAGGGGGAGAAAAAGGGAGTACCTTAAAAGTAGACGTCCCGGTATTCACTCT  •N  S  D  D  P  E  D  R  W  S  F  V  V  F  C  L  R  I  A  V• 241 TAACAGTGATGACCCAGAAGATAGATGGAGCTTTGTGGTATTCTGCCTCCGGATTGCTGT  •S  E  D  A  N  K  P  L  R  Q  G  A  L  I  S  L  L  C  S  H• 301 TAGCGAAGATGCCAACAAACCACTCAGGCAAGGTGCTCTCATATCTCTTTTATGCTCCCA  •S  Q  V  M  R  N  H  V  A  I  A  G  K  Q  N  E  A  T  L  A• 361 CTCACAGGTAATGAGGAACCATGTTGCCATTGCAGGGAAACAGAATGAAGCCACATTGGC  •V  L  E  I  D  G  F  A  N  G  T  P  Q  F  N  N  R  S  G  V• 421 CGTGCTTGAGATTGATGGCTTTGCCAACGGCACGCCCCAGTTCAACAATAGGAGTGGAGT  •S  E  E  R  A  Q  R  F  A  M  I  A  G  S  L  P  R  A  C  S• 481 GTCTGAAGAGAGAGCACAGAGATTTGCGATGATAGCAGGATCTCTCCCTCGGGCATGCAG  •N  G  T  P  F  V  T  A  G  A  E  D  D  A  P  E  D  I  T  D• 541 CAACGGAACCCCGTTCGTCACAGCCGGGGCAGAAGATGATGCACCAGAAGACATCACCGA  •T  L  E  R  I  L  S  I  Q  A  Q  V  W  V  T  V  A  K  A  M• 601 TACCCTGGAGAGGATCCTCTCTATCCAGGCTCAAGTATGGGTCACAGTAGCAAAAGCCAT  •T  A  Y  E  T  A  D  E  S  E  T  R  R  I  N  K  Y  M  Q  Q• 661 GACTGCGTATGAGACTGCAGATGAGTCGGAAACAAGGCGAATCAATAAGTATATGCAGCA  •G  R  V  Q  K  K  Y  I  L  Y  P  V  C  R  S  T  I  Q  L  T• 721 AGGCAGGGTCCAAAAGAAATACATCCTCTACCCCGTATGCAGGAGCACAATCCAACTCAC  •I  R  Q  S  L  A  V  R  I  F  L  V  S  E  L  K  R  G  R  N• 781 GATCAGACAGTCTCTTGCAGTCCGCATCTTTTTGGTTAGCGAGCTCAAGAGAGGCCGCAA  •T  A  G  G  T  S  T  Y  Y  N  L  V  G  D  V  D  S  Y  I  R• 841 CACGGCAGGTGGTACCTCTACTTATTATAACCTGGTAGGGGACGTAGACTCATACATCAG  •N  T  G  L  T  A  F  F  L  T  L  K  Y  G  I  N  T  K  T  S• 901 GAATACCGGGCTTACTGCATTCTTCTTGACACTCAAGTACGGAATCAACACCAAGACATC  •A  L  A  L  S  S  L  S  G  D  I  Q  K  M  K  Q  L  M  R  L• 961 AGCCCTTGCACTTAGTAGCCTCTCAGGCGACATCCAGAAGATGAAGCAGCTCATGCGTTT  •Y  R  M  K  G  D  N  A  P  Y  M  T  L  L  G  D  S  D  Q  M• 1021 GTATCGGATGAAAGGAGATAATGCGCCGTACATGACATTACTTGGTGATAGTGACCAGAT  •S  F  A  P  A  E  Y  A  Q  L  Y  S  F  A  M  G  M  A  S  V• 1081 GAGCTTTGCGCCTGCCGAGTATGCACAACTTTACTCCTTTGCCATGGGTATGGCATCAGT  •L  D  K  G  T  G  K  Y  Q  F  A  R  D  F  M  S  T  S  F  W• 1141 CCTAGATAAAGGTACTGGGAAATACCAATTTGCCAGGGACTTTATGAGCACATCATTCTG  •R  L  G  V  E  Y  A  Q  A  Q  G  S  S  I  N  E  D  M  A  A• 1201 GAGACTTGGAGTAGAGTACGCTCAGGCTCAGGGAAGTAGCATTAACGAGGATATGGCTGC  •E  L  K  L  T  P  A  A  M  K  G  L  A  A  A  A  Q  R  V  S• 1261 CGAGCTAAAGCTAACCCCAGCAGCAATGAAGGGCCTGGCAGCTGCTGCCCAACGGGTCTC  •D  D  T  S  S  I  Y  M  P  T  Q  Q  V  G  V  L  T  G  L  S• 1321 CGACGATACCAGCAGCATATACATGCCTACTCAACAAGTCGGAGTCCTCACTGGGCTTAG  •E  G  G  S  Q  A  L  Q  G  G  S  N  R  S  Q  G  Q  P  E  A• 1381 CGAGGGGGGGTCCCAAGCTCTACAAGGCGGATCGAATAGATCGCAAGGGCAACCAGAAGC  •G  D  G  E  T  Q  F  L  D  L  M  R  A  V  A  N  S  M  R  E• 1441 CGGGGATGGGGAGACCCAATTCCTGGATCTGATGAGAGCGGTAGCAAATAGCATGAGGGA  •A  P  N  S  A  Q  G  T  P  Q  S  G  P  P  P  T  P  G  P  S• 1501 GGCGCCAAACTCTGCACAGGGCACTCCCCAATCGGGGCCTCCCCCAACTCCTGGGCCATC  •Q  D  N  D  T  D  W  G  Y 1561 CCAAGATAACGACACCGACTGGGGGTATTGAtggacaaaacccagcctgcttccacaaaa 1621 acatcccaatgccctcacccgtagtcgacccctcgatttgcggctctatatgaccacacc 1681 ctcaaacaaacatccccctctttcctccctccccctgctgtacaactccgcacgccctag                                                    gene End 1741 ataccacaggcacaatgcggctcactaacaatcaaaacagagccgagggaa ttagaaaaa    gene start 1801 a gt acgggtagaa gagggatattcagagatcagggcaagtctcccgagtctctgctctct                            M  A  T  F  T  D  A  E  I  D  E  L 1861 cctctacctgatagaccaggacaaacATGGCCACCTTTACAGATGCAGAGATCGACGAGC  • F  E  T  S  G  T  V  I  D  N  I  I  T  A  Q  G  K  P  A  E• 1921 TATTTGAGACAAGTGGAACTGTCATTGACAACATAATTACAGCCCAGGGTAAACCAGCAG  • T  V  G  R  S  A  I  P  Q  G  K  T  K  V  L  S  A  A  W  E• 1981 AGACTGTTGGAAGGAGTGCAATCCCACAAGGCAAGACCAAGGTGCTGAGCGCAGCATGGG  • K  H  G  S  I  Q  P  P  A  S  Q  D  N  P  D  R  Q  D  R  S• 2041 AGAAGCATGGGAGCATCCAGCCACCGGCCAGTCAAGACAACCCCGATCGACAGGACAGAT  • D  K  Q  P  S  T  P  E  Q  T  T  P  H  D  S  P  P  A  T  S• 2101 CTGACAAACAACCATCCACACCCGAGCAAACGACCCCGCATGACAGCCCGCCGGCCACAT  • A  D  Q  P  P  T  Q  A  T  D  E  A  V  D  T  Q  F  R  T  G• 2161 CCGCCGACCAGCCCCCCACCCAGGCCACAGACGAAGCCGTCGACACACAGTTCAGGACCG  • A  S  N  S  L  L  L  M  L  D  K  L  S  N  K  S  S  N  A  K• 2221 GAGCAAGCAACTCTCTGCTGTTGATGCTTGACAAGCTCAGCAATAAATCGTCCAATGCTA     ApaI  • K  G  P  W  S  S  P  Q  E  G  N  H  Q  R  P  T  Q  Q  Q  G• 2281 AAAA GGGCCC ATGGTCGAGCCCCCAAGAGGGGAATCACCAACGTCCGACTCAACAGCAGG  • S  Q  P  S  R  G  N  S  Q  E  R  P  Q  N  Q  V  K  A  A  P• 2341 GGAGTCAACCCAGTCGCGGAAACAGTCAGGAAAGACCGCAGAACCAAGTCAAGGCCGCCC  • G  N  Q  G  T  D  V  N  T  A  Y  H  G  Q  W  E  E  S  Q  L• 2401 CTGGAAACCAGGGCACAGACGTGAACACAGCATATCATGGACAATGGGAGGAGTCACAAC  • S  A  G  A  T  P  H  A  L  R  S  R  Q  S  Q  D  N  T  L  V• 2461 TATCAGCTGGTGCAACCCCTCATGCTCTCCGATCAAGGCAGAGCCAAGACAATACCCTTG  • S  A  D  H  V  Q  P  P  V  D  F  V  Q  A  M  M  S  M  M  E• 2521 TATCTGCGGATCATGTCCAGCCGCCTGTAGACTTTGTGCAAGCGATGATGTCTATGATGG  • A  I  S  Q  R  V  S  K  V  D  Y  Q  L  D  L  V  L  K  Q  T• 2581 AGGCGATATCACAGAGAGTAAGTAAGGTTGACTATCAGCTAGATCTTGTCTTGAAACAGA  • S  S  I  P  M  M  R  S  E  I  Q  Q  L  K  T  S  V  A  V  M• 2641 CATCCTCCATCCCTATGATGCGGTCCGAAATCCAACAGCTGAAAACATCTGTTGCAGTCA  • E  A  N  L  G  M  M  K  I  L  D  P  G  C  A  N  I  S  S  L• 2701 TGGAAGCCAACTTGGGAATGATGAAGATTCTGGATCCCGGTTGTGCCAACATTTCATCTC  • S  D  L  R  A  V  A  R  S  H  P  V  L  V  S  G  P  G  D  P• 2761 TGAGTGATCTACGGGCAGTTGCCCGATCTCACCCGGTTTTAGTTTCAGGCCCTGGAGACC  • S  P  Y  V  T  Q  G  G  E  M  A  L  N  K  L  S  Q  P  V  P• 2821 CCTCTCCCTATGTGACACAAGGAGGCGAAATGGCACTTAATAAACTTTCGCAACCAGTGC  • H  P  S  E  L  I  K  P  A  T  A  C  G  P  D  I  G  V  E  K• 2881 CACATCCATCTGAATTGATTAAACCCGCCACTGCATGCGGGCCTGATATAGGAGTGGAAA  • D  T  V  R  A  L  I  M  S  R  P  M  H  P  S  S  S  A  K  L• 2941 AGGACACTGTCCGTGCATTGATCATGTCACGCCCAATGCACCCGAGTTCTTCAGCCAAGC  • L  S  K  L  D  A  A  G  S  I  E  E  I  R  K  I  K  R  L  A• 3001 TCCTAAGCAAGTTAGATGCAGCCGGGTCGATCGAGGAAATCAGGAAAATCAAGCGCCTTG  • L  N  G 3061 CTCTAAATGGCTAAttactactgccacacgtagcgggtccctgtccactcggcatcacac 3121 ggaatctgcaccgagttcccccccgcagacccaaggtccaactctccaagcggcaatcct 3181 ctctcgcttcctcagccccactgaatgatcgcgtaaccgtaattaatctagctacattta     Gene End   Gene start                         M  D  S  S• 3241 agat taagaaaaaa t acgggtagaa ttggagtgccccaattgtgccaagATGGACTCATC  • R  T  I  G  L  Y  F  D  S  A  H  S  S  S  N  L  L  A  F  P• 3301 TAGGACAATTGGGCTGTACTTTGATTCTGCCCATTCTTCTAGCAACCTGTTAGCATTTCC  • I  V  L  Q  D  T  G  D  G  K  K  Q  I  A  P  Q  Y  R  I  Q• 3361 GATCGTCCTACAAGACACAGGAGATGGGAAGAAGCAAATCGCCCCGCAATATAGGATCCA  • R  L  D  L  W  T  D  S  K  E  D  S  V  F  I  T  T  Y  G  F• 3421 GCGCCTTGACTTGTGGACTGATAGTAAGGAGGACTCAGTATTCATCACCACCTATGGATT  • I  F  Q  V  G  N  E  E  A  T  V  G  M  I  D  D  K  P  K  R• 3481 CATCTTTCAAGTTGGGAATGAAGAAGCCACTGTCGGCATGATCGATGATAAACCCAAGCG                            AvrII  • E  L  L  S  A  A  M  L  C  L  G  S  V  P  N  T  G  D  L  I• 3541 CGAGTTACTTTCCGCTGCGATGCTCTG CCTAGG AAGCGTCCCAAATACCGGAGACCTTAT  • E  L  A  R  A  C  L  T  M  I  V  T  C  K  K  S  A  T  N  T• 3601 TGAGCTGGCAAGGGCCTGTCTCACTATGATAGTCACATGCAAGAAGAGTGCAACTAATAC  • E  R  M  V  F  S  V  V  Q  A  P  Q  V  L  Q  S  C  R  V  V• 3661 TGAGAGAATGGTTTTCTCAGTAGTGCAGGCACCCCAAGTGCTGCAAAGCTGTAGGGTTGT  • A  N  K  Y  S  S  V  N  A  V  K  H  V  K  A  P  E  K  I  P• 3721 GGCAAACAAATACTCATCAGTGAATGCAGTCAAGCACGTGAAAGCGCCAGAGAAGATTCC  • G  S  G  T  L  E  Y  K  V  N  F  V  S  L  T  V  V  P  K  K• 3781 CGGGAGTGGAACCCTAGAATACAAGGTGAACTTTGTCTCCTTGACTGTGGTACCGAAGAA  • D  V  Y  K  I  P  A  A  V  L  K  V  S  G  S  S  L  Y  N  L• 3841 GGATGTCTACAAGATCCCAGCTGCAGTATTGAAGGTTTCTGGCTCGAGTCTGTACAATCT  • A  L  N  V  T  I  N  V  E  V  D  P  R  S  P  L  V  K  S  L• 3901 TGCGCTCAATGTCACTATTAATGTGGAGGTAGACCCGAGGAGTCCTTTGGTTAAATCTCT  • S  K  S  D  S  G  Y  Y  A  N  L  F  L  H  I  G  L  M  T  T• 3961 GTCTAAGTCTGACAGCGGATACTATGCTAACCTCTTCTTGCATATTGGACTTATGACCAC  • V  D  R  K  G  K  K  V  T  F  D  K  L  E  K  K  I  R  S  L• 4021 CGTAGATAGGAAGGGGAAGAAAGTGACATTTGACAAGCTGGAAAAGAAAATAAGGAGCCT  • D  L  S  V  G  L  S  D  V  L  G  P  S  V  L  V  K  A  R  G• 4081 TGATCTATCTGTCGGGCTCAGTGATGTGCTCGGGCCTTCCGTGTTGGTAAAAGCAAGAGG  • A  R  T  K  L  L  A  P  F  F  S  S  S  G  T  A  C  Y  P  I• 4141 TGCACGGACTAAGCTTTTGGCACCTTTCTTCTCTAGCAGTGGGACAGCCTGCTATCCCAT  • A  N  A  S  P  Q  V  A  K  I  L  W  S  Q  T  A  C  L  R  S• 4201 AGCAAATGCTTCTCCTCAGGTGGCCAAGATACTCTGGAGTCAAACCGCGTGCCTGCGGAG  • V  K  I  I  I  Q  A  G  T  Q  R  A  V  A  V  T  A  D  H  E• 4261 CGTTAAAATCATTATCCAAGCAGGTACCCAACGCGCTGTCGCAGTGACCGCCGACCACGA  • V  T  S  T  K  L  E  K  G  H  T  L  A  K  Y  N  P  F  K  K• 4321 GGTTACCTCTACTAAGCTGGAGAAGGGGCACACCCTTGCCAAATACAATCCTTTTAAGAA  • 4381 ATAAgctgcgtctctgagattgcgctccgcccactcacccagatcatcatgacacaaaaa                                               Gene End   MluI 4441 actaatctgtcttgattatttacagttagtttacctgtctatcaag ttagaaaaaa c acg                                                    M  G  S  K•    Gene start 4501 cgtacgggtagaa gagtctggatcccgaccggcacattcaggacgcaatATGGGCTCCAA  •L  S  T  R  I  P  A  P  L  M  L  T  T  R  I  T  L  I  L  S• 4561 ACTTTCTACCAGGATTCCAGCACCTCTGATGCTGACCACCCGGATTACGCTGATATTGAG  • C  I  R  P  T  S  S  L  D  G  R  P  L  A  A  A  G  I  V  V• 4621 CTGTATCCGTCCGACAAGCTCTCTTGACGGCAGGCCTCTTGCAGCTGCAGGAATTGTAGT  • T  G  D  K  A  V  N  V  Y  T  S  S  Q  T  G  S  I  I  V  K• 4681 AACAGGAGATAAGGCAGTCAATGTATACACCTCGTCTCAGACAGGGTCAATCATAGTCAA  • L  L  P  N  M  P  R  D  K  E  A  C  A  K  A  P  L  E  A  Y• 4741 GTTGCTCCCGAATATGCCCAGGGATAAAGAGGCGTGTGCAAAAGCCCCATTAGAGGCATA  • N  R  T  L  T  T  L  L  T  P  L  G  D  S  I  R  K  I  Q  G• 4801 TAACAGAACACTGACTACTTTGCTAACTCCTCTTGGCGACTCCATCCGCAAGATCCAAGG                  Cleavage site  • S  V  S  T  S  G  G  G  R  Q  A  R  L  I  G  A  V  I  G  S• 4861 GTCTGTGTCCACGTCTGGAGGAGGCAGACAAGCACGCCTGATAGGTGCTGTTATTGGCAG                                         NotI  • V  A  L  G  V  A  T  A  A  Q  I  T  A  A  A  A  L  I  Q  A• 4921 TGTAGCTCTTGGGGTTGCAACAGCGGCACAGATAACAGCA GCGGCCGC CCTAATACAAGC  • N  Q  N  A  A  N  I  L  R  L  K  E  S  I  A  A  T  N  E  A• 4981 CAACCAGAATGCCGCCAACATCCTCCGGCTTAAGGAGAGCATTGCTGCAACCAATGAAGC  • V  H  E  V  T  D  G  L  S  Q  L  S  V  A  V  G  K  M  Q  Q• 5041 TGTGCATGAAGTCACCGACGGATTATCACAACTATCAGTGGCAGTTGGGAAGATGCAGCA  • F  V  N  D  Q  F  N  N  T  A  R  E  L  D  C  I  K  I  T  Q• 5101 GTTCGTCAATGACCAGTTTAATAATACAGCACGAGAATTGGACTGTATAAAAATCACACA  • Q  V  G  V  E  L  N  L  Y  L  T  E  L  T  T  V  F  G  P  Q• 5161 ACAGGTTGGTGTAGAGCTAAACCTATACCTAACTGAATTGACTACAGTATTCGGGCCACA  • I  T  S  P  A  L  T  Q  L  T  I  Q  A  L  Y  N  L  A  G  G• 5221 GATCACTTCCCCTGCATTAACTCAGTTGACCATCCAAGCACTTTATAATTTAGCTGGTGG  • N  M  N  Y  L  L  T  K  L  G  I  G  N  N  Q  L  S  S  L  I• 5281 CAATATGAATTACTTATTAACTAAGTTAGGTATAGGGAACAATCAACTCAGCTCATTAAT  • G  S  G  L  I  T  G  Y  P  I  L  Y  D  S  Q  T  Q  L  L  G• 5341 TGGTAGCGGCCTGATCACTGGTTACCCTATACTGTATGATTCACAGACTCAACTCTTGGG  • I  Q  V  N  L  P  S  V  G  N  L  N  N  M  R  A  T  Y  L  E• 5401 CATACAAGTGAATTTGCCCTCAGTCGGGAACTTAAATAATATGCGTGCCACCTATTTGGA  • T  L  S  V  S  T  T  K  G  Y  A  S  A  L  V  P  K  V  V  T• 5461 GACCTTATCTGTAAGTACAACCAAAGGATATGCCTCAGCACTTGTCCCGAAAGTAGTGAC  • Q  V  G  S  V  I  E  E  L  D  T  S  Y  C  I  E  S  D  L  D• 5521 ACAGGTCGGTTCTGTGATAGAAGAGCTCGACACCTCATACTGCATAGAGTCCGATCTGGA  • L  Y  C  T  R  I  V  T  F  P  M  S  P  G  I  Y  S  C  L  S• 5581 TTTATATTGTACTAGAATAGTGACATTCCCCATGTCCCCAGGTATTTATTCCTGCTTGAG  • G  N  T  S  A  C  M  Y  S  K  T  E  G  A  L  T  T  P  Y  M• 5641 CGGCAACACATCAGCTTGCATGTATTCAAAGACTGAAGGCGCACTCACTACGCCGTATAT  • A  L  K  G  S  V  I  A  N  C  K  I  T  T  C  R  C  T  D  P• 5701 GGCCCTTAAAGGCTCGGTTATTGCCAATTGTAAGATAACAACATGTAGATGTACAGACCC  • P  G  I  I  S  Q  N  Y  G  E  A  V  S  L  I  D  R  H  S  C• 5761 TCCTGGTATCATATCGCAAAATTATGGAGAAGCCGTATCCCTGATAGATAGACATTCGTG  • N  V  L  S  L  D  G  I  T  L  R  L  S  G  E  F  D  A  T  Y• 5821 CAATGTCTTATCATTAGACGGGATAACTCTGAGGCTCAGTGGGGAATTTGATGCAACTTA  • Q  K  N  I  S  I  L  D  S  Q  V  I  V  T  G  N  L  D  I  S• 5881 TCAAAAGAACATCTCAATACTAGATTCTCAAGTCATCGTGACAGGCAATCTTGATATCTC  • T  E  L  G  N  V  N  N  S  I  S  N  A  L  D  S  L  A  E  S• 5941 AACTGAACTTGGAAACGTCAACAATTCAATCAGCAATGCCTTGGATAGTTTGGCAGAAAG  • N  S  K  L  E  K  I  N  V  R  L  T  S  T  S  A  L  I  T  Y• 6001 CAACAGGAAGCTGGAAAAAATCAATGTCAGACTAACCAGCACATCTGCTCTCATTACCTA  • I  V  L  T  V  I  S  L  V  F  G  A  F  S  L  G  L  A  C  Y• 6061 TATTGTTCTAACTGTCATTTCTCTAGTTTTCGGTGCATTTAGTTTGGGTTTAGCGTGTTA  • L  M  Y  K  Q  K  A  Q  Q  K  T  L  L  W  L  G  N  N  T  L• 6121 CCTGATGTACAAACAGAAGGCACAACAAAAGACCTTGCTATGGCTTGGGAATAATACCCT  • D  Q  M  R  A  T  T  R  A 6181 CGATCAGATGAGAGCCACTACAAGAGCATGAatgcagataagaggtgggtatatacccaa                                              Gene End 6241 cagcagcctgtgtatcaattccgataacctgtcaagtagaagact taagaaaaaa ctact           Gene start 6301 gggaataagcaaccaaagagcactac acgggtagaa cgatcagaggagccacccttcaat                                                           M 6361 cggaaattaggcttcacaacatccgttctaccgcatcaccaacaacaagagtcaatcATG           HpaI  D  R  A  V  N  R  V  V  L  E  N  E  E  R  E  A  K  N  T  W 6421 GACCGCGCG GTTAAC AGAGTCGTGCTGGAGAATGAGGAAAGAGAAGCAAAGAACACATGG  R  L  V  F  R  I  A  V  L  L  L  M  V  M  T  L  A  I  S  S 6481 CGCCTGGTTTTCCGGATCGCAGTTTTACTTTTAATGGTAATGACTCTAGCTATCTCCTCA  A  A  L  A  Y  S  T  G  A  S  T  P  H  D  L  A  S  I  L  T 6541 GCTGCCCTGGCATACAGCACGGGGGCCAGTACGCCGCACGACCTCGCAAGCATATTGACT  V  I  S  K  T  E  D  K  V  T  S  L  L  S  S  S  Q  D  V  I 6601 GTGATCTCCAAGACAGAAGATAAGGTTACGTCTTTACTCAGTTCAAGTCAAGACGTGATA  D  R  I  Y  K  Q  V  A  L  E  S  P  L  A  L  L  N  T  E  S 6661 GATAGGATATACAAGCAGGTGGCTCTTGAATCCCCGCTGGCACTACTAAACACTGAATCT  V  I  M  N  A  I  T  S  L  S  Y  Q  I  N  G  A  A  N  N  S 6721 GTAATTATGAATGCAATAACCTCTCTTTCTTATCAAATTAACGGGGCTGCGAACAATAGC  G  C  G  A  P  V  H  D  P  D  Y  I  G  G  I  G  K  E  L  I 6781 GGATGTGGGGCGCCTGTTCATGACCCAGATTATATCGGGGGGATAGGCAAAGAACTCATA  V  D  D  I  S  D  V  T  S  F  Y  P  S  A  Y  Q  E  H  L  N 6841 GTGGACGACATCAGTGATGTTACATCATTTTATCCTTCTGCATATCAAGAACACTTGAAT  F  I  P  A  P  T  T  G  S  G  C  T  R  I  P  S  F  D  M  S 6901 TTCATCCCGGCACCTACTACAGGATCCGGTTGCACTCGGATACCCTCGTTTGACATGAGC  T  T  H  Y  C  Y  T  H  N  V  I  L  S  G  C  R  D  H  S  H 6961 ACCACCCATTATTGTTATACTCACAATGTGATACTATCCGGTTGCAGAGATCACTCACAC  S  H  Q  Y  L  A  L  G  V  L  R  T  S  A  T  G  R  V  F  F 7021 TCACATCAATACTTAGCACTTGGTGTGCTTCGGACATCTGCAACAGGGAGGGTATTCTTT  S  T  L  R  S  I  N  L  D  D  T  Q  N  R  K  S  C  S  V  S 7081 TCTACTCTGCGCTCTATCAATTTAGATGACACCCAAAATCGGAAGTCCTGCAGTGTGAGT  A  T  P  L  G  C  D  M  L  C  S  K  V  T  G  T  E  E  E  D 7141 GCAACCCCTTTAGGTTGTGATATGCTGTGCTCCAAGGTCACAGGGACTGAAGAGGAGGAT Y  K  S  V  A  P  T  S  M  V  H  G  R  L  G  F  D  G  Q  Y 7201 TACAAGTCAGTTGCCCCCACATCAATGGTGCACGGAAGGCTAGGGTTTGACGGTCAATAC H  E  K  D  L  D  T  T  V  L  F  K  D  W  V  A  N  Y  P  G 7261 CATGAAAAGGACTTAGACACCACGGTCTTATTTAAGGATTGGGTGGCAAATTACCCAGGA  A  G  G  G  S  F  I  D  D  R  V  W  F  P  V  Y  G  G  L  K 7321 GCGGGAGGAGGGTCTTTTATTGACGACCGTGTATGGTTCCCAGTTTACGGAGGGCTCAAA  P  D  S  P  S  D  T  A  Q  E  G  K  Y  V  I  Y  K  R  H  N 7381 CCCGATTCACCCAGTGACACTGCACAAGAAGGGAAATACGTAATATACAAGCGCCATAAC  N  T  C  P  D  K  Q  D  Y  Q  I  R  K  A  K  S  S  Y  K  P 7441 AACACATGCCCCGATAAACAAGATTACCAAATTCGGAAGGCTAAGTCTTCATATAAACCC  G  R  F  G  G  K  R  V  Q  Q  A  I  L  S  I  K  V  S  T  S 7501 GGGCGATTTGGTGGGAAGCGCGTACAGCAAGCCATCTTATCCATCAAAGTGTCAACATCT  L  G  K  D  P  V  L  T  I  P  P  N  T  I  T  L  M  G  A  E 7561 TTGGGTAAGGACCCGGTGCTGACTATTCCACCTAATACAATCACACTCATGGGAGCCGAA  G  R  I  L  T  V  G  T  S  H  F  L  Y  Q  R  G  S  S  Y  F 7621 GGCAGAATTCTCACAGTGGGGACATCTCACTTCTTGTACCAACGAGGGTCTTCATATTTC  S  P  A  L  L  Y  P  M  T  V  N  N  K  T  A  T  L  H  S  P 7681 TCCCCTGCCTTATTATATCCCATGACAGTAAATAACAAAACGGCTACACTCCATAGTCCT  Y  T  F  N  A  F  T  R  P  G  S  V  P  C  Q  A  S  A  R  C 7741 TATACGTTTAATGCTTTCACTCGGCCAGGTAGTGTCCCTTGCCAGGCATCAGCAAGATGC  P  N  S  C  I  T  G  V  Y  T  D  P  Y  P  L  I  F  H  R  N 7801 CCCAACTCATGCATTACTGGAGTCTATACTGATCCATATCCCTTAATCTTCCATAGGAAT  H  T  L  R  G  V  F  G  T  M  L  D  D  E  Q  A  R  L  N  P 7861 CATACTCTACGAGGGGTCTTCGGAACGATGCTTGATGATGAACAAGCGAGACTTAACCCC  V  S  A  V  F  D  N  V  S  R  S  R  V  T  R  V  S  S  S  S 7921 GTATCCGCAGTATTCGACAACGTATCCCGCAGTCGTGTCACCCGGGTGAGTTCAAGCAGC  T  K  A  A  Y  T  T  S  T  C  F  K  V  V  K  T  N  K  T  Y 7981 ACCAAGGCAGCATACACGACATCGACATGTTTCAAAGTTGTCAAGACCAATAAAACTTAT  C  L  S  I  A  E  I  S  N  T  L  F  G  E  F  R  I  V  P  L 8041 TGTCTTAGTATTGCAGAAATATCCAATACCCTGTTCGGGGAAATTTAGGATCGTTCCCTT A SpeI  L  V  E  I  L  K  D  D  G  V  R  E  A  R  S  G 8101 CTAGT TGAGATCCTCAAGGATGACGGGGTTAGAGAAGCCAGGTCTGGCTAGttgagtcaa 8161 ttataaaggagttggaaagatggcattgtatcacctatcttctgtgacatcaagaatcaa 8221 accgaatgccggcgcgtgctcgaattccatgttgccagttgaccacaatcagccagtgct                                       Gene End 8281 catgcgatcagattaagccttgtcaatagtctcttgat taagaaaaaa tgtaagtggcaa                                     Gene start M  A  S  S  G                                     8341 tgagatacaaggcaaaacagctcatggtaaataat acgggtagga cATGGCGAGCTCCGG  •P  E  R  A  E  H  Q  I  I  L  P  E  S  H  L  S  S  P  L  V• 8401 TCCTGAAAGGGCAGAGCATCAGATTATCCTACCAGAGTCACACCTGTCTTCACCATTGGT  •K  H  K  L  L  Y  Y  W  K  L  T  G  L  P  L  P  D  E  C  D• 8461 CAAGCACAAACTACTCTATTACTGGAAATTAACTGGGCTACCGCTTCCTGATGAATGTGA  •F  D  H  L  I  L  S  R  Q  W  K  K  I  L  E  S  A  S  P  D• 8521 CTTCGACCACCTCATTCTCAGCCGACAATGGAAAAAAATACTTGAATCGGCCTCTCCTGA  •T  E  R  M  I  K  L  G  R  A  V  H  Q  T  L  N  H  N  S  R• 8581 TACTGAGAGAATGATAAAACTCGGAAGGGCAGTACACCAAACTCTTAACCACAATTCCAG  •I  T  G  V  L  H  P  R  C  L  E  E  L  A  N  I  E  V  P  D• 8641 AATAACCGGAGTGCTCCACCCCAGGTGTTTAGAAGAACTGGCTAATATTGAGGTCCCAGA  •S  T  N  K  F  R  K  I  E  K  K  I  Q  I  H  N  T  R  Y  G• 8701 TTCAACCAACAAATTTCGGAAGATTGAGAAGAAGATCCAAATTCACAACACGAGATATGG  •E  L  F  T  R  L  C  T  H  I  E  K  K  L  L  G  S  S  W  S• 8761 AGAACTGTTCACAAGGCTGTGTACGCATATAGAGAAGAAACTGCTGGGGTCATCTTGGTC                                      BsiWI  •N  N  V  P  R  S  E  E  F  S  S  I  R  T  D  P  A  F  W  F• 8821 TAACAATGTCCCCCGGTCAGAGGAGTTCAGCAGCATT CGTACG GATCCGGCATTCTGGTT  •H  S  K  W  S  T  A  K  F  A  W  L  H  I  K  Q  I  Q  R  H• 8881 TCACTCAAAATGGTCCACAGCCAAGTTTGCATGGCTCCATATAAAACAGATCCAGAGGCA  •L  M  V  A  A  R  T  R  S  A  A  N  K  L  V  M  L  T  H  K• 8941 TCTGATGGTGGCAGCTAGGACAAGGTCTGCGGCCAACAAATTGGTGATGCTAACCCATAA  •V  G  Q  V  F  V  T  P  E  L  V  V  V  T  H  T  N  E  N  K• 9001 GGTAGGCCAAGTCTTTGTCACTCCTGAACTTGTCGTTGTGACGCATACGAATGAGAACAA  •F  T  C  L  T  Q  E  L  V  L  M  Y  A  D  M  M  E  G  R  D• 9061 GTTCACATGTCTTACCCAGGAACTTGTATTGATGTATGCAGATATGATGGAGGGCAGAGA  •M  V  N  I  I  S  T  T  A  V  H  L  R  S  L  S  E  K  I  D• 9121 TATGGTCAACATAATATCAACCACGGCGGTGCATCTCAGAAGCTTATCAGAGAAAATTGA  •D  I  L  R  L  I  D  A  L  A  K  D  L  G  N  Q  V  Y  D  V• 9181 TGACATTTTGCGGTTAATAGACGCTCTGGCAAAAGACTTGGGTAATCAAGTCTACGATGT  •V  S  L  M  E  G  F  A  Y  G  A  V  Q  L  L  E  P  S  G  T• 9241 CGTATCACTAATGGAGGGATTTGCATACGGAGCTGTCCAGCTACTCGAGCCGTCAGGTAC  •F  A  G  D  F  F  A  F  N  L  Q  E  L  K  D  I  L  I  G  L• 9301 ATTTGCAGGAGATTTCTTCGCATTCAACCTGCAGGAGCTTAAAGACATTCTAATTGGCCT  •L  P  N  D  I  A  E  S  V  T  H  A  I  A  T  V  F  S  G  L• 9361 CCTCCCCAATGATATAGCAGAATCCGTGACTCATGCAATCGCTACTGTATTCTCTGGTTT  •E  Q  N  Q  A  A  E  M  L  C  L  L  R  L  W  G  H  P  L  L• 9421 AGAACAGAATCAAGCAGCTGAGATGTTGTGTCTGTTGCGTCTGTGGGGTCACCCACTGCT  •E  S  R  I  A  A  K  A  V  R  S  Q  M  C  A  P  K  M  V  D• 9481 TGAGTCCCGTATTGCAGCAAAGGCAGTCAGGAGCCAAATGTGCGCACCGAAAATGGTAGA  •F  D  M  I  L  Q  V  L  S  F  F  K  G  T  I  I  N  G  Y  R• 9541 CTTTGATATGATCCTTCAGGTACTGTCTTTCTTCAAGGGAACAATCATCAACGGGTACAG  •K  K  N  A  G  V  W  P  R  V  K  V  D  T  I  Y  G  K  V  I• 9601 AAAGAAGAATGCAGGTGTGTGGCCGCGAGTCAAAGTGGATACAATATATGGGAAGGTCAT  •G  Q  L  H  A  D  S  A  E  I  S  H  D  I  M  L  R  E  Y  K• 9661 TGGGCAACTACATGCAGATTCAGCAGAGATTTCACACGATATCATGTTAAGAGAGTATAA  •S  L  S  A  L  E  F  E  P  C  I  E  Y  D  P  V  T  N  L  S• 9721 GAGTTTATCTGCACTTGAATTTGAGCCATGTATAGAATATGACCCTGTCACCAACCTGAG  •M  F  L  K  D  K  A  I  A  H  P  N  D  N  W  L  A  S  F  R• 9781 CATGTTCCTAAAAGACAAGGCAATCGCACACCCCAACGATAATTGGCTTGCCTCGTTTAG  •R  N  L  L  S  E  D  Q  K  K  H  V  K  E  A  T  S  T  N  R• 9841 GCGGAACCTTCTCTCCGAAGACCAGAAGAAACATGTAAAAGAAGCAACTTCGACTAATCG  •L  L  I  E  F  L  E  S  N  D  F  D  P  Y  K  E  M  E  Y  L• 9901 CCTCTTGATAGAGTTTTTAGAGTCAAATGATTTTGATCCATATAAAGAGATGGAATATCT  •T  T  L  E  Y  L  R  D  D  N  V  A  V  S  Y  S  L  K  E  K• 9961 GACGACCCTTGAGTACCTTAGAGATGACAATGTGGCAGTATCATACTCGCTCAAGGAGAA  •E  V  K  V  N  G  R  I  F  A  K  L  T  K  K  L  R  N  C  Q• 10021 GGAAGTGAAAGTTAATGGACGGATCTTCGCTAAGCTGACAAAGAAGTTAAGGAACTGTCA   •V  M  A  E  G  I  L  A  D  Q  I  A  P  F  F  Q  G  N  G V• 10081 GGTGATGGCGGAAGGGATCCTAGCCGATCAGATTGCACCTTTCTTTCAGGGAAATGGAGT   •I  Q  D  S  I  S  L  T  K  S  M  L  A  M  S  Q  L  S  F N• 10141 CATTCAGGATAGCATATCCTTGACCAAGAGTATGCTAGCGATGAGTCAACTGTCTTTTAA   •S  N  K  K  R  I  T  D  C  K  E  R  V  S  S  N  R  N  H D• 10201 CAGCAATAAGAAACGTATCACTGACTGTAAAGAAAGAGTATCTTCAAACCGCAATCATGA   •P  K  S  K  N  R  R  R  V  A  T  F  I  T  T  D  L  Q  K Y• 10261 TCCGAAAAGCAAGAACCGTCGGAGAGTTGCAACCTTCATAACAACTGACCTGCAAAAGTA   •C  L  N  W  R  Y  Q  T  I  K  L  F  A  H  A  I  N  Q  L M• 10321 CTGTCTTAATTGGAGATATCAGACAATCAAATTGTTCGCTCATGCCATCAATCAGTTGAT   •G  L  P  H  F  F  E  W  I  H  L  R  L  M  D  T  T  M  F V• 10381 GGGCCTACCTCACTTCTTCGAATGGATTCACCTAAGACTGATGGACACTACGATGTTCGT   •G  D  P  F  N  P  P  S  D  P  T  D  C  D  L  S  R  V  P N• 10441 AGGAGACCCTTTCAATCCTCCAAGTGACCCTACTGACTGTGACCTCTCAAGAGTCCCTAA   •D  D  I  Y  I  V  S  A  R  G  G  I  E  G  L  C  Q  K  L W• 10501 TGATGACATATATATTGTCAGTGCCAGAGGGGGTATCGAAGGATTATGCCAGAAGCTATG   •T  M  I  S  I  A  A  I  Q  L  A  A  A  R  S  H  C  R  V A• 10561 GACAATGATCTCAATTGCTGCAATCCAACTTGCTGCAGCTAGATCGCATTGTCGTGTTGC   •C  M  V  Q  G  D  N  Q  V  I  A  V  T  R  E  V  R  S  D D• 10621 CTGTATGGTACAGGGTGATAATCAAGTAATAGCAGTAACGAGAGAGGTAAGATCAGACGA   •S  P  E  M  V  L  T  Q  L  H  Q  A  S  D  N  F  F  K  E L• 10681 CTCTCCGGAGATGGTGTTGACACAGTTGCATCAAGCCAGTGATAATTTCTTCAAGGAATT   •I  H  V  N  H  L  I  G  H  N  L  K  D  R  E  T  I  R  S D• 10741 AATTCATGTCAATCATTTGATTGGCCATAATTTGAAGGATCGTGAAACCATCAGGTCAGA   •T  F  F  I  Y  S  K  R  I  F  K  D  G  A  I  L  S  Q  V L• 10801 CACATTCTTCATATACAGCAAACGAATCTTCAAAGATGGAGCAATCCTCAGTCAAGTCCT   •K  N  S  S  K  L  V  L  V  S  G  D  L  S  E  N  T  V  M S• 10861 CAAAAATTCATCTAAATTAGTGCTAGTGTCAGGTGATCTCAGTGAAAACACCGTAATGTC   •C  A  N  I  A  S  T  V  A  R  L  C  E  N  G  L  P  K  D F• 10921 CTGTGCCAACATTGCCTCTACTGTAGCACGGCTATGCGAGAACGGGCTTCCCAAAGACTT   •C  Y  Y  L  N  Y  I  M  S  C  V  Q  T  Y  F  D  S  E  F S• 10981 CTGTTACTATTTAAACTATATAATGAGTTGTGTGCAGACATACTTTGACTCTGAGTTCTC   •I  T  N  N  S  H  P  D  L  N  Q  S  W  I  E  D  I  S  F V• 11041 CATCACCAACAATTCGCACCCCGATCTTAATCAGTCGTGGATTGAGGACATCTCTTTTGT   •H  S  Y  V  L  T  P  A  Q  L  G  G  L  S  N  L  Q  Y  S R• 11101 GCACTCATATGTTCTGACTCCTGCCCAATTAGGGGGACTGAGTAACCTTCAATACTCAAG   •L  Y  T  R  N  I  G  D  P  G  T  T  A  F  A  E  I  K  R L• 11161 GCTCTACACTAGAAATATCGGTGACCCGGGGACTACTGCTTTTGCAGAGATCAAGCGACT   •E  A  V  G  L  L  S  P  N  I  M  T  N  I  L  T  R  P  P G• 11221 AGAAGCAGTGGGATTACTGAGTCCTAACATTATGACTAATATCTTAACTAGGCCGCCTGG   •N  G  D  W  A  S  L  C  N  D  P  Y  S  F  N  F  E  T  V A• 11281 GAATGGAGATTGGGCCAGTCTGTGCAACGACCCATACTCTTTCAATTTTGAGACTGTTGC   •S  P  N  I  V  L  K  K  H  T  Q  R  V  L  F  E  T  C  S N• 11341 AAGCCCAAATATTGTTCTTAAGAAACATACGCAAAGAGTCCTATTTGAAACTTGTTCAAA   •P  L  L  S  G  V  H  T  E  D  N  E  A  E  E  K  A  L  A E• 11401 TCCCTTATTGTCTGGAGTGCACACAGAGGATAATGAGGCAGAAGAGAAGGCATTGGCTGA   •F  L  L  N  Q  E  V  I  H  P  R  V  A  H  A  I  M  E  A S• 11461 ATTCTTGCTTAATCAAGAGGTGATTCATCCCCGCGTTGCGCATGCCATCATGGAGGCAAG   •S  V  G  R  R  K  Q  I  Q  G  L  V  D  T  T  N  T  V  I K• 11521 CTCTGTAGGTAGGAGAAAGCAAATTCAAGGGCTTGTTGACACAACAAACACCGTAATTAA   •I  A  L  T  R  R  P  L  G  I  K  R  L  M  R  I  V  N  Y S• 11581 GATTGCGCTTACTAGGAGGCCATTAGGCATCAAGAGGCTGATGCGGATAGTCAATTATTC   •S  M  H  A  M  L  F  R  D  D  V  F  S  S  S  R  S  N  H P• 11641 TAGCATGCATGCAATGCTGTTTAGAGACGATGTTTTTTCCTCCAGTAGATCCAACCACCC   •L  V  S  S  N  M  C  S  L  T  L  A  D  Y  A  R  N  R  S W• 11701 CTTAGTCTCTTCTAATATGTGTTCTCTGACACTGGCAGACTATGCACGGAATAGAAGCTG   •S  P  L  T  G  G  R  K  I  L  G  V  S  N  P  D  T  I  E L• 11761 GTCACCTTTGACGGGAGGCAGGAAAATACTGGGTGTATCTAATCCTGATACGATAGAACT   •V  E  G  E  I  L  S  V  S  G  G  C  T  R  C  D  S  G  D E• 11821 CGTAGAGGGTGAGATTCTTAGTGTAAGCGGAGGGTGTACAAGATGTGACAGCGGAGATGA   •Q  F  T  W  F  H  L  P  S  N  I  E  L  T  D  D  T  S  K N• 11881 ACAATTTACTTGGTTCCATCTTCCAAGCAATATAGAATTGACCGATGACACCAGCAAGAA   •P  P  M  R  V  P  Y  L  G  S  K  T  Q  E  R  R  A  A  S L• 11941 TCCTCCGATGAGGGTACCATATCTCGGGTCAAAGACACAGGAGAGGAGAGCTGCCTCACT   •A  K  I  A  H  M  S  P  H  V  K  A  A  L  R  A  S  S  V L• 12001 TGCAAAAATAGCTCATATGTCGCCACATGTAAAGGCTGCCCTAAGGGCATCATCCGTGTT   •I  W  A  Y  G  D  N  E  V  N  W  T  A  A  L  T  I  A  K S• 12061 GATCTGGGCTTATGGGGATAATGAAGTAAATTGGACTGCTGCTCTTACGATTGCAAAATC   •R  C  N  V  N  L  E  Y  L  R  L  L  S  P  L  P  T  A  G N• 12121 TCGGTGTAATGTAAACTTAGAGTATCTTCGGTTACTGTCCCCTTTACCCACGGCTGGGAA   •L  Q  H  R  L  D  D  G  I  T  Q  M  T  F  T  P  A  S  L Y• 12181 TCTTCAACATAGACTAGATGATGGTATAACTCAGATGACATTCACCCCTGCATCTCTCTA   •R  V  S  P  Y  I  H  I  S  N  D  S  Q  R  L  F  T  E  E G• 12241 CAGGGTGTCACCTTACATTCACATATCCAATGATTCTCAAAGGCTGTTCACTGAAGAAGG   •V  K  E  G  N  V  V  Y  Q  Q  I  M  L  L  G  L  S  L  I E• 12301 AGTCAAAGAGGGGAATGTGGTTTACCAACAGATCATGCTCTTGGGTTTATCTCTAATCGA ClaI   •S  I  F  P  M  T  T  T  R  T  Y  D  E  I  T  L  H  L  H S• 12361 ATCGAT ATTTCCAATGACAACAACCAGGACATATGATGAGATCACACTGCACCTACATAG   •K  F  S  C  C  I  R  E  A  P  V  A  V  P  F  E  L  L  G V• 12421 TAAATTTAGTTGCTGTATCAGAGAAGCACCTGTTGCGGTTCCTTTCGAGCTACTTGGGGT   •V  P  E  L  R  T  V  T  S  N  K  F  M  Y  D  P  S  P  V S• 12481 GGTACCGGAACTGAGGACAGTGACCTCAAATAAGTTTATGTATGATCCTAGCCCTGTATC   •E  G  D  F  A  R  L  D  L  A  I  F  K  S  Y  E  L  N  L E• 12541 GGAGGGAGACTTTGCGAGACTTGACTTAGCTATCTTCAAGAGTTATGAGCTTAATCTGGA   •S  Y  P  T  I  E  L  M  N  I  L  S  I  S  S  G  K  L  I G• 12601 GTCATATCCCACGATAGAGCTAATGAACATTCTTTCAATATCCAGCGGGAAGTTGATTGG   •Q  S  V  V  S  Y  D  E  D  T  S  I  K  N  D  A  I  I  V Y• 12661 CCAGTCTGTGGTTTCTTATGATGAAGATACCTCCATAAAGAATGACGCCATAATAGTGTA   •D  N  T  R  N  W  I  S  E  A  Q  N  S  D  V  V  R  L  F E• 12721 TGACAATACCCGAAATTGGATCAGTGAAGCTCAGAATTCAGATGTGGTCCGCCTATTTGA   •Y  A  A  L  E  V  L  L  D  C  S  Y  Q  L  Y  Y  L  R  V R• 12781 ATATGCAGCACTTGAAGTGCTCCTCGACTGTTCTTACCAACTCTATTACCTGAGAGTAAG   •G  L  D  N  I  V  L  Y  M  G  D  L  Y  K  N  M  P  G  I L• 12841 AGGCCTAGACAATATTGTCTTATATATGGGTGATTTATACAAGAATATGCCAGGAATTCT   •L  S  N  I  A  A  T  I  S  H  P  V  I  H  S  R  L  H  A V• 12901 ACTTTCCAACATTGCAGCTACAATATCTCATCCCGTCATTCATTCAAGGTTACATGCAGT   •G  L  V  N  H  D  G  S  H  Q  L  A  D  T  D  F  I  E  M S• 12961 GGGCCTGGTCAACCATGACGGATCACACCAACTTGCAGATACGGATTTTATCGAAATGTC   •A  K  L  L  V  S  C  T  R  R  V  I  S  G  L  Y  S  G  N K• 13021 TGCAAAACTATTAGTATCTTGCACCCGACGTGTGATCTCCGGCTTATATTCAGGAAATAA   •Y  D  L  L  F  P  S  V  L  D  D  N  L  N  E  K  M  L  Q L• 13081 GTATGATCTGCTGTTCCCATCTGTCTTAGATGATAACCTGAATGAGAAGATGCTTCAGCT   •I  S  R  L  C  C  L  Y  T  V  L  F  A  T  T  R  E  I  P K• 13141 GATATCCCGGTTATGCTGTCTGTACACGGTACTCTTTGCTACAACAAGAGAAATCCCGAA   •I  R  G  L  T  A  E  E  K  C  S  I  L  T  E  Y  L  L  S D• 13201 AATAAGAGGCTTAACTGCAGAAGAGAAATGTTCAATACTCACTGAGTATTTACTGTCGGA   •A  V  K  P  L  L  S  P  D  Q  V  S  S  I  M  S  P  N  I I• 13261 TGCTGTGAAACCATTACTTAGCCCCGATCAAGTGAGCTCTATCATGTCTCCTAACATAAT   •T  F  P  A  N  L  Y  Y  M  S  R  K  S  L  N  L  I  R  E R• 13321 TACATTCCCAGCTAATCTGTACTACATGTCTCGGAAGAGCCTCAATTTGATCAGGGAAAG   •E  D  R  D  T  I  L  A  L  L  F  P  Q  E  P  L  L  E  F P• 13381 GGAGGACAGGGATACTATCCTGGCGTTGTTGTTCCCCCAAGAGCCATTATTAGAGTTCCC   •S  V  Q  D  I  G  A  R  V  K  D  P  F  T  R  Q  P  A  A F• 13441 TTCTGTGCAAGATATTGGTGCTCGAGTGAAAGATCCATTCACCCGACAACCTGCGGCATT   •L  Q  E  L  D  L  S  A  P  A  R  Y  D  A  F  T  L  S  Q I• 13501 TTTGCAAGAGTTAGATTTGAGTGCTCCAGCAAGGTATGACGCATTCACACTTAGTCAGAT   •H  P  E  L  T  S  P  N  P  E  E  D  Y  L  V  R  Y  L  F R• 13561 TCATCCTGAACTCACATCTCCAAATCCGGAGGAAGACTACTTAGTACGATACTTGTTCAG   •G  I  G  T  A  S  S  S  W  Y  K  A  S  H  L  L  S  V  P E• 13621 AGGGATAGGGACTGCATCTTCCTCTTGGTATAAGGCATCCCATCTCCTTTCTGTACCCGA   •V  R  C  A  R  H  G  N  S  L  Y  L  A  E  G  S  G  A  I M• 13681 GGTAAGATGTGCAAGACACGGGAACTCCTTATACTTAGCTGAAGGGAGCGGAGCCATCAT   •S  L  L  E  L  H  V  P  H  E  T  I  Y  Y  N  T  L  F  S N• 13741 GAGTCTTCTCGAACTGCATGTACCACATGAAACTATCTATTACAATACGCTCTTTTCAAA   •E  M  N  P  P  Q  R  H  F  G  P  T  P  T  Q  F  L  N  S V• 13801 TGAGATGAACCCCCCGCAACGACATTTCGGGCCGACCCCAACTCAGTTTTTGAATTCGGT   •V  Y  R  N  L  Q  A  E  V  T  C  K  D  G  F  V  Q  E  F R• 13861 TGTTTATAGGAATCTACAGGCGGAGGTAACATGCAAAGATGGATTTGTCCAAGAGTTCCG   •P  L  W  R  E  N  T  E  E  S  D  L  T  S  D  K  A  V  G Y• 13921 TCCATTATGGAGAGAAAATACAGAGGAAAGTGACCTGACCTCAGATAAAGCAGTGGGGTA   •I  T  S  A  V  P  Y  R  S  V  S  L  L  H  C  D  I  E  I P• 13981 TATTACATCTGCAGTGCCCTACAGATCTGTATCATTGCTGCATTGTGACATTGAAATTCC   •P  G  S  N  Q  S  L  L  D  Q  L  A  I  N  L  S  L  I  A M• 14041 TCCAGGGTCCAATCAAAGCTTACTAGATCAACTAGCTATCAATTTATCTCTGATTGCCAT   •H  S  V  R  E  G  G  V  V  I  I  K  V  L  Y  A  M  G  Y Y• 14101 GCATTCTGTAAGGGAGGGCGGGGTAGTAATCATCAAAGTGTTGTATGCAATGGGATACTA   •F  H  L  L  M  N  L  F  A  P  C  S  T  K  G  Y  I  L  S N• 14161 CTTTCATCTACTCATGAACTTGTTTGCTCCGTGTTCCACAAAAGGATATATTCTCTCTAA   •G  Y  A  C  R  G  D  M  E  C  Y  L  V  F  V  M  G  Y  L  G• 14221 TGGTTATGCATGTCGAGGAGATATGGAGTGTTACCTGGTATTTGTCATGGGTTACCTGGG   •G  P  T  F  V  H  E  V  V  R  M  A  K  T  L  V  Q  R  H  G• 14281 CGGGCCTACATTTGTACATGAGGTGGTGAGGATGGCAAAAACTCTGGTGCAGCGGCACGG   •T  L  L  S  K  S  D  E  I  T  L  T  R  L  F  T  S  Q  R  Q• 14341 TACGCTTTTGTCTAAATCAGATGAGATCACACTGACCAGGTTATTCACCTCACAGCGGCA   •R  V  T  D  I  L  S  S  P  L  P  R  L  I  K  Y  L  R  K  N• 14401 GCGTGTGACAGACATCCTATCCAGTCCTTTACCAAGATTAATAAAGTACTTGAGGAAGAA   •I  D  T  A  L  I  E  A  G  G  Q  P  V  R  P  F  C  A  E  S• 14461 TATTGACACTGCGCTGATTGAAGCCGGGGGACAGCCCGTCCGTCCATTCTGTGCGGAGAG   •L  V  S  T  L  A  N  I  T  Q  I  T  Q  I  I  A  S  H  I  D• 14521 TCTGGTGAGCACGCTAGCGAACATAACTCAGATAACCCAGATCATCGCTAGTCACATTGA   •T  V  I  R  S  V  I  Y  M  E  A  E  G  D  L  A  D  T  V  F• 14581 CACAGTTATCCGGTCTGTGATATATATGGAAGCTGAGGGTGATCTCGCTGACACAGTATT   •L  F  T  P  Y  N  L  S  T  D  G  K  K  R  T  S  L  K  Q  C• 14641 TCTATTTACCCCTTACAATCTCTCTACTGACGGGAAAAAGAGGACATCACTTAAACAGTG   •T  R  Q  I  L  E  V  T  I  L  G  L  R  V  E  N  L  N  K  I• 14701 CACGAGACAGATCCTAGAGGTTACAATACTAGGTCTTAGAGTCGAAAATCTCAATAAAAT   •G  D  I  I  S  L  V  L  K  G  M  I  S  M  E  D  L  I  P  L• 14761 AGGCGATATAATCAGCCTAGTGCTTAAAGGCATGATCTCCATGGAGGACCTTATCCCACT                                                 AvrII   •R  T  Y  L  K  H  S  T  C  P  K  Y  L  K  A  V  L  G  I  T• 14821 AAGGACATACTTGAAGCATAGTACCTGCCCTAAATATTTGAAGGCTGT CCTAGG TATTAC   •K  L  K  E  M  F  T  D  T  S  V  L  Y  L  T  R  A  Q  Q  K• 14881 CAAACTCAAAGAAATGTTTACAGACACTTCTGTACTGTACTTGACTCGTGCTCAACAAAA   •F  Y  M  K  T  I  G  N  A  V  K  G  Y  Y  S  N  C  D  S 14941 ATTCTACATGAAAACTATAGGCAATGCAGTCAAAGGATATTACAGTAACTGTGACTCTTA 15001 Acgaaaatcacatattaataggctccttttttggccaattgtattcttgttgatttaatc         Gene End 15061 atattatg ttagaaaaaa gttgaaccctgactccttaggactcgaattcgaactcaaata 15121 aatgtcttaaaaaaaggttgcgcacaattattcttgagtgtagtctcgtcattcaccaaa         HDV ribozyme sequences 15181 tctttgtttggtGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCG 15241 AAGGAGGACGTCGTCCACTCGGATGGCTAAGGGAGAGCtcggatccggctgctaacaaag                               T7 terminator 15301 cccgaaaggaagctgagttggctgctgccaccgctgagcaataaCTAGCATAACCCCTTG 15361 GGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGcatatgcggtgtgaaataccgcacagat 15421 gcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgc 15481 gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttat 15541 ccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca 15601 ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc 15661 atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacc 15721 aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg 15781 gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta 15841 ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccg 15901 ttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagac 15961 acgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtag 16021 gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat 16081 ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgat 16141 ccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgc 16201 gcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt 16261 ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacct 16321 agatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaactt 16381 ggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc 16441 gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttac 16501 catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat 16561 cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg 16621 cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaata 16681 gtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggta 16741 tggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgt 16801 gcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcag 16861 tgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaa 16921 gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggc 16981 gaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactt 17041 taaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgc 17101 tgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta 17161 ctttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaa 17221 taagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagca 17281 tttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaac 17341 aaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccatta 17401 ttatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaat      T7 Promoter 17461 TCTAATACGACTCACTATAGG

The amino acid sequences of NP, P, M, F, HN and L proteins expressed by the vector for transcription of the genome (pTNH-c4152R2L) are shown in SEQ ID NO: 2 to 7, respectively.

In another aspect, the present invention relates to a recombinant Newcastle disease virus including coding regions of P, M, F, HM and L gene, wherein the coding regions of the NP, P, M and L genes are derived from a low-pathogenic Newcastle disease virus, and the coding regions of the F and HN genes are derived from a high-pathogenic Newcastle disease virus, wherein the F protein coding sequence is characterized by substitution of the codon for the 115^(th) amino acid of the F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU.

The HN gene of the recombinant Newcastle disease virus may be additionally mutated, so that the codons for the 1^(st) to 569^(th) amino acids of the HN protein encodes those of a high-pathogenic Newcastle disease virus and the codons for the amino acids after the 570^(th) position encode those of a low-pathogenic Newcastle disease virus including the La Sota strain.

In a preferred embodiment of the present invention, the recombinant Newcastle disease virus may be KCTC10984BP.

The recombinant Newcastle disease virus according to the present invention is characterized in that the surface antigen and antigenicity are identical or similar to those of high-pathogenic field strains, and the pathogenicity is lower than that of existing low-pathogenic vaccine strains. The pathogenic Newcastle disease virus has the furin recognizing cleavage region in the F protein, and when the cleavage region is cleaved by furin, the fusion peptide region where the F protein is fused with cellular membrane is exposed, thereby obtaining infectivity. Since furin is distributed in most cells in the body, the Newcastle disease virus can be systemically infected in the body, thereby showing high pathogenicity.

In the vector for transcription of the virus genome of the present invention, the low-pathogenic Newcastle disease virus is the same as defined above, for example, the low-pathogenic Newcastle disease virus may be selected from the group consisting of Newcastle disease viruses belonging to Type I or Type II, and preferably may be the La Sota/46 strain (AY845400) belonging to the Type II. The high-pathogenic Newcastle disease virus is the same as defined above, it may be any one selected from the group consisting of Newcastle disease viruses belonging to Type V, Type VI, Type VII, Type VIII, and Type XI, which are field strains prevailing throughout the world, and is preferably selected from the group consisting of Newcastle disease viruses belonging to Type VI and Type VII. In a preferred embodiment of the present invention, the high-pathogenic Newcastle disease virus may be KBNP-4152 (deposition No, KCTC10919BP). A method of preparing the KBNP-4152 strain and characteristics thereof are the same as described in Korean Patent Application No. 2006-0026667 and 'Cho S H, Aim Y J, Kim S J, Kwon H J. Characterization of a Newcastle disease virus with variation in the major Hemagglutinin-Neuraminidase (HN) linear epitope. The 49th Annual Meeting of the Korean Society of Veterinary Science 2005, 45 (3, suppl), 199', which are incorporated herein by reference). As used herein, the high-pathogenic Newcastle disease viruses and the low-pathogenic Newcastle disease viruses are defined as described above, as long as they are differently defined.

In the attenuation of the Newcastle disease virus using conventional reverse genetics, there is an example of substituting the 115^(th) amino acid with glycine, but any mutation at only one base of the codon for the substituted glycine can lead to the alteration of the 115^(th) amino acid to a basic amino acid such as lysine or arginine, and the virus may recover its pathogenicity again.

However, in the recombinant Newcastle disease virus according to the present invention, the codon encoding the 115^(th) amino acid of the F protein is substituted with the codon encoding a non-basic amino acid, wherein the codon encoding the non-basic amino acid cannot be converted into one encoding a basic amino acid until at least two point mutations occur therein. Therefore, the probability of the recombinant virus recovers its pathogenicity is very low, and the stability of the virus is significantly increased compared with existing other attenuated strains. When the cleavage site of the F protein is mutated according to the present invention, the cleavage site of the F protein is cleaved by not furin, whereby a systemic infection cannot occur, but trypsin or trypsin-like enzymes distributed in only a few organs in the body, respiratory organs and digestive organs, resulting in a localized infection.

In order to achieve such effect, the codon for the 115^(th) amino acid of the F protein of the present invention is substituted with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU.

In another aspect, the present invention relates to a method of preparing a recombinant Newcastle disease virus, including the steps of:

substituting nucleotide sequences encoding the F and HN protein of a low-pathogenic Newcastle disease virus with those of a high-pathogenic Newcastle disease virus; and substituting the codon for the 115^(th) amino acid of F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU,

wherein the virus is characterized by having antigenicity equal or similar to the high-pathogenic Newcastle disease virus and decreased pathogenicity.

In preparing the recombinant Newcastle disease virus, the HN gene may be additionally mutated so that the codons for the 1^(st) to 569^(th) amino acids of the HN protein encodes those of a high-pathogenic Newcastle disease virus and the codons for the amino acids after the 570^(th) position encode those of a low-pathogenic Newcastle disease virus including the La Sota strain.

In addition, the method includes the steps of: transfecting the above-described vector for transcription of the Newcastle disease virus genome according to the present invention into a host cell; and rescuing the recombinant Newcastle disease virus. The host cell used in the transfection is not particularly limited, and preferably may be an animal cell selected from the group consisting of Hep2 and BHK21.

In another aspect, the present invention relates to a method of attenuating the pathogenicity and improving the antigenicity and stability of the Newcastle disease virus, including the steps of:

substituting nucleotide sequences encoding the F and HN protein of a low-pathogenic Newcastle disease virus with those of a high-pathogenic Newcastle disease virus; and

substituting the codon encoding the 115^(th) amino acid of F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU.

In the method, the HN gene of the vector may be additionally mutated so that the codons for the 1^(st) to 569^(th) amino acids of the HN protein encodes those of a high-pathogenic Newcastle disease virus and the codons for the amino acids after 570^(th) position encode those of a low-pathogenic Newcastle disease virus including the La Sota strain.

In another aspect, the present invention relates to a Newcastle disease vaccine containing the recombining Newcastle disease virus with an increased antigenicity and a decreased pathogenicity as described above. The Newcastle disease vaccine may be an inactivated vaccine obtained by inactivating the recombining Newcastle disease virus. The inactivation may be performed by using any conventional means known to the technical field to which this invention belongs, for example, using formaldehyde or bromomethyl amine hydrobromide, etc. Alternatively, it is also possible to use the Newcastle disease vaccine in the form of a live vaccine, or an in ovo vaccine applicable directly to a fertilized egg, due to the low pathogenicity, high stability and high safety of the recombining Newcastle disease virus. When the vaccine of the present invention is used in the form of a live vaccine, its administration pathway is not limited, and for example, it may be administered through subcutaneous or muscular pathway, or by means of spray or drinking water, appropriately for symptoms and purposes. The dose of the vaccine of the present invention may depend on the administration method, and the condition of subject to be administered, and for example, the dose of the vaccine may be from 10¹ EID₅₀ (50% egg-infectious dose) to 10¹² EID₅₀ strain/individual. More specifically, in the case of the inactivated vaccine, the vaccine may be preferably used in the amount of 10^(6.0˜12) EID₅₀/individual, and more preferably 10^(8.0˜10) EID₅₀/individual. In the case of the in ovo vaccine, it may be used in the amount of 10^(1˜9.0) EID₅₀/egg according to the level of maternal antibodies in the egg to be administered, and more preferably, in the amount of 10^(3.0˜7.0) EID₅₀/egg.

As described above, the pathogenicity of the Newcastle disease virus is determined by the amino acid sequence of the furin recognizing cleavage region of the F protein. That is, when the amino acid sequence of the furin recognizing cleavage region of the F protein is R-X-K/R-R (positioning from 113^(th) to 116^(th) positions; hereinafter, the position range is applied to all amino acid tetramers, unless differently defined), the F protein is cleaved and activated by furin existing in all cells of whole body, resulting in a systemic infection, thereby showing high pathogenicity. On the contrary, when there is only one basic amino acid, or discontinuous basic amino acids, such as R-Q-G-R or G-Q-G-R, the F protein is activated by trypsin or analogs thereof existing in parts of the epithelial cells of the digestive tract and trachea only, resulting in a localized infection, thereby showing low pathogenicity. As to HN, a highly pathogenic virus consists of 571 amino acids, which is a relatively short amino acid length. On the contrary, a low pathogenic virus consists of 577 or 616 amino acids, which is a relatively long amino acid length, wherein the additional C-terminal region makes it possible to distinguish the low pathogenic virus from the high pathogenic virus. Therefore, it is possible to prepare viruses with various levels of pathogenicities by preparing recombinant viruses with various combinations of the cleavage site of the F protein and the additional C-terminal region of HN.

Particularly, the present invention is characterized in that the amino acid sequence of the cleavage site of the F protein is substituted by a safer amino acid sequence, in order to inhibit the recombinant virus from obtaining pathogenicity by any possible genetic variations. As described above, the amino acid sequence of the cleavage site of the F protein of a low pathogenic virus is R-Q-G-R or K-Q-G-R. In order for the low pathogenic virus to be modified into a high pathogenic virus, the above amino acid sequence should be changed into R-X-K/R-R, and thus, the glycine (G) located at the third position has to be changed into arginine (R) or lysine (K). Such change from G to R or K may readily occur by only one point mutation. That is, considering that the codon for glycine is GGA, GGC, GGG or GGU, and the codon for arginine or lysine is AGA, AGG, CGA, CGC, CGG, CGU, AAA or AAG, glycine may be easily modified to arginine or lysine even if only one point mutation occur in any of the glycine codons, resulting converting a low pathogenic virus into a high pathogenic one. In fact, it has been reported in Australia in 2001 that a non-pathogenic Newcastle disease virus, Ulster like strain, became pathogenic through a similar mechanism to the above, thereby causing Newcastle disease.

Therefore, in order to remarkably decrease the probability of the increase of pathogenicity caused by any point mutation in the vaccine strain compared with the existing strain, the present invention provides a technique to prepare a recombinant virus using any conventional method, such as a PTDS (PCR-based-two-steps DNA synthesis) method, wherein the recombinant virus shows a low probability of such amino acid change into lysine or arginine caused by point mutation. That is, in the present invention, an amino acid, glycine, located at the 115^(th) position of the F protein of a conventional low-pathogenic virus is substituted by any one selected from the group consisting of alanine, aspartic acid, phenylalanine, isoleucine, leucine, serine, theronine, valine and tyrosine, for example resulting in R-Q-A-R or G-Q-A-R, wherein the amino acid that is substituted for the 115^(th) glycine can be changed to lysine or arginine only when all or at least two bases are mutated in the codon for the amino acid to be substituted for the 115^(th) glycine, allowing preparation of a safer vaccine strain.

The level of pathogenicity of the recombinant Newcastle disease virus is determined by measuring mean death time (MDT), and intracerebral pathogenicity index (ICPI). The biological properties of the recombinant Newcastle disease virus is confirmed by EID₅₀ (50% egg-infectious dose), hemagglutination dissociation rate, and the like. As a result, the recombinant Newcastle disease virus according to the present invention has remarkably lower pathogenicity than the existing low pathogenic strains.

The present invention is further explained in more detail with reference to the following examples. These examples, however, should not be interpreted as limiting the scope of the present invention in any manner.

EXAMPLE Example 1 Cloning of a Virus Gene

1.1. Synthesis of a Virus cDNA

Recently, as a virus representing the Newcastle disease viruses, a velogenic and antigenic variant strain that is domestically prevalent, the SNU4152 strain that was isolated in the Avian Disease & Laboratory, College of Veterinary Medicine of Seoul National University, was selected. The virus was cloned after plaque-purifying 3 times on chicken embryonic fibroblast (CEF), and was sub-cultivated twice in an SPF embryonated egg. The cloned strain was named KBNP-4152 and deposited with the Korean Collection for Type Culture (Korea Research Institute of Bioscience and Biotechnology, Taejon, Republic of Korea) on Mar. 10, 2006, and assigned deposition No. KCTC10919BP.

RNA manipulation was performed in a glass and a plastic ware without RNase, and all solutions were used to triple distilled water (DEPC-DW) that was autoclaved and treated with 1% diethyl-pyrocarbonate (DEPC). Then, the virus was centrifuged at 21,000 rpm for 70 min in a Beckman SW40 rotor, and an obtained pellet was re-suspended in a buffer solution (50 mM Tris HCl pH7.5, 50 mM EDTA, 0.5% SDS). After treating with protease K (200 μg/ml, Invitrogen Co.) at 37° C. for 90 min, RNA was extracted by acidic phenol extraction. Then, after precipitating the RNA by ethanol precipitation, the obtained precipitate was washed with 75% ethanol, dried, and re-suspended in DEPC-treated water.

1 μ

of the extracted RNA quantified by ultraviolet spectrometer (Eppendorf, Biophotometer), 1 μ

of primer (10 pmol/μ

) shown in Table 1, and 10 μ

of DEPC-water were mixed, and the mixture was denaturated at 70° C. for 10 min. After adding 4 μ

of 5×RT buffer solution (250 mM Tris-HCl, pH 8.3, 375 mM KCl, 15 mM MgCl₂; GibcoBRL/Life Technologies), 2 μ

of 0.1 M DTT, and 2 μ

of 10 mM dNTPs (each 2.5 mM) to the mixture, the mixture was reacted at 42° C. for 2 min. Then, 1 μ

of reverse transcriptase (200 unit, Invitrogen co.) was added to the reacted mixture, which was then reacted at 42° C. for 60 min.

TABLE 1 Primers used for synthesis of KBNP-4152 cDNA Primer Primer sequence (5′->3′) ND-ZJ-1F cgtctcgaccaaacagagaatctgtgaggtac (SEQ ID NO: 8) ND-ZJ-1746F gacaacacaggcacagctcg (SEQ ID NO: 9) ND-ZJ-2827F catctccttacgtgacacaagg (SEQ ID NO: 10) ND-ZJ-F-F tcgcgacgcaatatgg ctccaaactt tc (SEQ ID NO: 11) ND-ZJ-HN-F ccgcggcaccgacaac aagagtcaat catg (SEQ ID NO: 12) ND-ZJ-8100F actagttgagatcctcaaggatgatag (SEQ ID NO: 13) ND-ZJ-11648F catgcaatgttgtccagagatg (SEQ ID NO: 14) ND-ZJ-12539F tcagagagagatttcgcgagac (SEQ ID NO: 15) ND-ZJ-14021F cattgtgacattgagattcctcc (SEQ ID NO: 16)

1.2. Cloning of a Virus Gene

Based on previous GenBank data, the 9 kinds of synthesized virus cDNA corresponding to 15,192 bp nucleotide sequences were amplified by PCR reaction using 9 primer sets shown in Table 2, and cloned. The obtained clones were named Z1 to Z9.

TABLE 2 Primers used for cloning of KBNP-4152 viral gene Primer Primer sequence (5′->3′) ND-ZJ-1F cgtctcgaccaaacagagaatctgtgaggtac (SEQ ID NO: 8) ND-ZJ-1844R tcgtcttggtctctggatgtctc (SEQ ID NO: 17) ND-ZJ-1746F gacaacacaggcacagctcg (SEQ ID NO: 9) ND-ZJ-2948R cttctccactcccatgtcagg (SEQ ID NO: 18) ND-ZJ-2827F catctccttacgtgacacaagg (SEQ ID NO: 10) ND-ZJ-4612R cagcataatccgggtgatcagc (SEQ ID NO: 19) ND-ZJ-F-F tcgcgacgcaatatggctccaaactttc (SEQ ID NO: 11) ND-ZJ-F-R ccgcggtagaacggatgttgtgaagcctaa (SEQ ID NO: 20) ND-ZJ-HN-F ccgcggcaccgacaacaagagtcaatcatg (SEQ ID NO: 12) ND-ZJ-HN-R ctcaacta

taagggaacgatcctaaattcc (SEQ ID NO: 21) ND-ZJ-8100F actagttgagatcctcaaggatgatag (SEQ ID NO: 13) ND-ZJ-11815R tatggtatcagggttggatacacc (SEQ ID NO: 22) ND-ZJ-11648F catgcaatgttgtccagagatg (SEQ ID NO: 14) ND-ZJ-12591R agctcataactcttgaagatagc (SEQ ID NO: 23) ND-ZJ-12539F tcagagagagatttcgcgagac (SEQ ID NO: 15) ND-ZJ-14110R cacagaatgcatggcaatcagg (SEQ ID NO: 24) ND-ZJ-14021F cattgtgacattgagattcctcc (SEQ ID NO: 16) ND-ZJ-15118R actgaatccgaatacgacttcc (SEQ ID NO: 25)

In order to decrease an artificial mutation during PCR reaction, Pwo polymerase (Invitrogen co.) with a DNA repairing function was used. Further, the PCR product was purified by a soluble purification kit (Boehringer Mannheim Co.) or by an agarose gel purification kit (Qiagen Co.).

The location of the products obtained by RT-PCR for the KBNP-4152 viral gene is shown in FIG. 1.

The 9 kinds of purified products were cloned into TA vector such as XL-Topo, pCR8/GW/Topo or pcDNA3.1V5 Topo vector (Invitrogen co.), and more than three clones were obtained, respectively. Then, the plasmid of the clones was prepared, and the nucleotide sequence of the plasmid was determined.

All nucleotide sequences were determined using a cyclic sequencing kit (PRISM Ready Reaction Dye terminator kit) and an automated DNA sequencer (ABI310, Applied Biosystems Co.).

The primer used for the nucleotide sequence analysis was an M13 forward primer and an M13 reverse primer, respectively. In the case of fragments that were not read by the primer, the nucleotide sequence was analyzed using primer shown in Table 3 according to a primer walking method.

TABLE 3 Primers used for analyzing a nucleotide sequence of KBNP-4152 virus Primer Primer sequence (5′->3′) ND-ZJ-597F ctgacactctggaaagaatcc (SEQ ID NO: 26) ND-ZJ-3421F gatccagcgc cttgattcgt (SEQ ID NO: 27) ND-ZJ-8662F caggtgtttagaagaactggc (SEQ ID NO: 28) ND-ZJ-5759F cctcctggtatcatatcgca (SEQ ID NO: 29) ND-ZJ-4679 gtaacaggagataaggcagtc (SEQ ID NO: 30) ND-ZJ-7670 ttcttgtaccaacgagggtc (SEQ ID NO: 31) ND-ZJ-9328F cctacaggagctcaaagacac (SEQ ID NO: 32) ND-ZJ-9977F ctaagagatgacagtgtggc (SEQ ID NO: 33) ND-ZJ-10588F acttgctgcagcaagatctc (SEQ ID NO: 34) ND-ZJ-13052F gtggtctcaggcttatatgc (SEQ ID NO: 35)

The TA-cloning vectors as described above are shown in FIG. 2B, and the size of fragments inserted in the vector that was treated with EcoRI is shown in FIG. 2A using PCR.

The KBNP-4152 strain showed the highest nucleotide sequence homology and amino acid sequence homology against SF02 derived from geese in China, and particularly in the case of NP, P, M, and L among the viral protein, the homology was more high than about 98%, but in the case of P, V, and HN, relatively low homology of about 97.6% and 95.0% was shown. The sequence homology results are shown in Table 4.

TABLE 4 Sequence homology between the KBNP-4152 strain and other stains NP P V M F HN L aa^(a) aa aa aa aa aa aa KBNP-4152 (nt^(b)) (nt) (nt) (nt) (nt) (nt) (nt) VII(SF02) 99.2 (97.1) 96.2 (97.6) 95.0 (96.8) 98.6 (98.0) 98.4 (97.6) 97.6 (97.6) 98.9 (98.0) (AF473851) VII(ZJ-1) 99.0 (97.0) 94.7 (97.1) 92.9 (95.7) 98.1 (97.4) 97.7 (97.5) 97.9 (97.7) 98.9 (98.0) (AF431744) Ulster 94.1 (87.2) 81.6 (83.1) 75.5 (79.0) 89.1 (86.6) 90.3 (86.2) 89.9 (84.4) 94.3 (87.2) (AY562991) La Sota 91.3 (84.5) 80.9 (81.6) 76.3 (78.6) 87.4 (84.7) 88.8 (84.2) 88.5 (81.7) 92.0 (85.9) (AY845400) ^(a)a percentage of amino acid sequence homology (%) ^(b)a percentage of nucleotide sequence homology (%)

Particularly, it may be considered that mutation on the V protein hindering the expression of interferon in cells is large, because the mutation of amino acid is accumulated in the process of overcoming defense system in the cells. Further, it may be considered that mutation on the HN protein is relatively large, because viruses having the mutation of a specific amino acid are selected in the process of avoiding humoral immune response as known in the mutation of linear antigen. The amino acid sequences and the nucleotide sequences of the each gene of the KBNP-4152 strain were registered in GenBank (Accession No. DQ839397). Besides, the change of cysteine residues and N-linked glycosylation sites of HN and F proteins, the change of amino acids affecting the structure and polarity of the HN protein, and the change of amino acids affecting structure and polarity of the F protein were measured. The changes are shown in Table 5 to 7.

TABLE 5 The change of cystine residues and N-liked glycosylation sites of HN and F protein HN F C1 (123) G5 (508) G6 (538) C2 (27) La Sota − (W) − + + SNU4152 + + + −(R) SNU0202 + + − −(R)

TABLE 6 The change of amino acids affecting structure and polarity of HN protein (508-510) 60 128 256 269 293 310 323 340 347 354 384 479 494 495 KBNP-4152 NVS L C G S K D D H K K K H D E VII(SL03) NIS S — — — — — N — — M — — — — VII(consensus) NIS S — — — — — N — E M — — — — Ulster SIS P — E — E N N Y E M E Y — K La Sota STS P W E R G S N Y E M E Y G V VGGA STS P W E R G S N Y E M E Y G — ^(a)Not done.

TABLE 7 The change of amino acids affecting structure and polarity of F protein 27 76 104 112 115 145 192 195 232 247 403 422 430 480 486 KBNP-4152 R C G R K N N R Q N D H D S S VII(consensus) — — — — — — — — — D — — — R — Ulster C F E G G — K Q — D — Q — K — La Sota C — E G G K K Q K D N Q G K R VGGA C — E G G K K Q K D N Q G K R

As shown in the results, it is confirmed that the KBNP-4152 strain used in the present invention has a genotype similar with genotype VII, and the strain has a genetic difference with other type of viruses including the previous vaccine strain, the La Sota strain.

Example 2 Preparation of a Recombinant Vector for Transcription of Newcastle Disease Virus (NDV) Genome Using La Sota Strain as a Backbone

2.1. Design and Construction of a Parental Vector (pTMH) of Expressing Newcastle Disease Virus (NDV)

For manufacturing a virus from NDV cDNA, the cDNA has to be transcribed as the same structure with the virus genome without adding an unnecessary base to both the 5′- and 3′-termini of the virus genome. To obtain this structure, a parental vector pTMH having features as follows was manufactured (SEQ ID NO: 84):

1) A T7 promoter was positioned in front of the transcriptional initiation site (refer to FIG. 1 and FIG. 4).

2) A hepatitis delta virus (HDV) ribozyme sequence was positioned at the back of NDV antigenomic sequence, to occur self cleavage.

3) A multi-cloning Site (MCS) used for cloning the NDV genome was finally positioned between the T7 promoter and the HDV ribozyme (refer to FIG. 4).

4) Used the origin of replication (ori) of pBR322, to make the cloning vector stablely exist in Escherichia coli, even though the whole NDV antigenome corresponding to about 15 kb nucleotide sequences was included.

5) two different restriction enzyme recognition sites, BsmB I and Bsa I were positioned between the T7 promoter and the 5′-terminus of the antigenome in which transcription of the NDV was initiated, and positioned between the HDV ribozyme and the 3′-terminus of the antigenome in which transcription of the NDV was terminated, specifically to make cause transcription of both terminals of the viral genome from the NDV antigenome.

As shown in FIG. 4, 1.5 pmol of each of the TM p2 primer and the TM p3 primer among the TM p1-p4 primers used for manufacturing the linker, 30 pmol of each of the TM p1 primer and the TM p4 primer, 5 μ

of 10×PCR buffer, 5 μ

of 2.5 mM dNTPs, and 2.5 U of Taq polymerase were mixed, and then DW was added to the mixture to make the total volume 50 μ

. Then, the mixture was reacted at 94° C. for 1 min, followed by 25 cycles at 90° C. for 30 sec, at 55° C. for 45 sec, and at 72° C. for 15 sec, and then further reacted at 72° C. for 5 min. After confirming the PCR amplicon, it was cloned into a pCR8/GW/Topo TA cloning vector, and the obtained clone having complete nucleotide sequences was named a pCR-TM vector.

By the PCR method using F and R primers of the HDV, and a pTV vector (received from Ph. D Park M H of Mogam Biotechnology Research Institute) as a template, the fragments including HDV ribozyme and T7 terminator regions were amplified, the obtained fragments were also cloned into a pCR/GW/Topo TA cloning vector, and the obtained clone having complete nucleotide sequences was named pCR-HDV.

The HDV fragment obtained by cutting the pCR-HDV vector with restriction enzymes, Bsa I and Nde I and the pCR-TM vector by cutting with the same restriction enzymes was ligated with T4 DNA ligase, which was transformed into a Top 10F′ competent cell. Then, the obtained transformed vector was name as pCR-TMH. For stablely cloning the vector to Escherichia coli, the T7 promoter-MCS-HDV ribozyme region of the pCR-TMH vector treated by restriction enzymes, EcoR I and Nde I was subcloned into a pBR322 vector (Promega Co., Cat. #D1511), and the obtained clone was named a pTMH vector (SEQ ID NO: 84).

A schematic diagram of a process of manufacturing the pTMH vector is shown in FIG. 5, a general cleavage map and nucleotide sequences of the parental vector pTMH are shown in FIG. 6, and nucleotide sequences of the manufactured pTMH vector are shown in FIG. 7.

In an embodiment of the present invention, the restriction enzyme recognition sites of the manufactured parental vector, pTMH are as follows:

(No site found) AccI AflII AgeI AvaI BclI BglII BsaAI BsaBI BsmF I BsmI BspMI BssHII BstBI BstEII Bsu36I EagI EcoRV HindIII HpaI KpnI Mfe I MluI NaeI NcoI NheI NotI NsiI PmeI PvuII SalI SfiI SmaI SphI XbaI Xcm I XhoI XmaI (One site found) XmnI 1959 SspI 2166 SpeI 64 SacII 55 SacI 175 RsrI 0 PstI 1605 NspI 471 NruI 49 NdeI 293 HincII 1903 FspI 1584 EcoRI 0 EaeI 1752 ClaI 43 BstXI 155 BsmB I 30 BsiWI 70 Bmr I 1404 BglI 1478 BanI 1312 BamHI 181 AvrII 76 AseI 1535 Apo I 0 ApaI 35 AlwNI 882 AflIII 471 (Two sites found) StyI 76, 256 RsaI 71, 1843 HaeII 345, 715 ApaLI 785, 2031 AatII 147, 2282 Bsa I 82, 1431*

2.2. Cloning of the Whole cDNA of La Sota Strain

RNA extraction and cDNA synthesis for the La Sota/46 strain (AY845400) were performed by the method according to Example 1.

2.2.1. PCR of NDV Full-Length cDNA

Based on GenBank data, the whole NDV cDNA corresponding to 15,186 bp nucleotide sequences was amplified by PCR reaction using a primer set shown in Table 8 by the method according to Example 1, and cloned.

TABLE 8 Primers used for cloning the whole genomic gene of the La Sota strain Primer Primer sequence (5′->3′) S1-F cgtctcgaccaaacagagaatccgtgagttacg (SEQ ID NO: 36) S1-R ccatgggccc tttttagcat tggacg (SEQ ID NO: 37) S2-F aaaagggccc atggtcgagc cc (SEQ ID NO: 38) S2-R tatcatcgat catgccgaca gtg (SEQ ID NO: 39) S3-F catgatcgat gataaaccca agc (SEQ ID NO: 40) S3-R tcgcgaatgagccggt cgggatccag ac (SEQ ID NO: 41) S4-F tcgcgacgcaatatgg ctccaaactt tc (SEQ ID NO: 42) S4-R ccgcggtagaacggat gttgtgaagc ctaa (SEQ ID NO: 43) S5-F ccgcggcaccgacaac aagagtcaat catg (SEQ ID NO: 44) S5-R ctcaacta

t aagggaacga tcctaaattc c (SEQ ID NO: 45) S6-F a

tagt tgagat cctcaaggat gatag (SEQ ID NO: 46) S7-R gatccgtacg aatgcagctg aactc (SEQ ID NO: 47) S9-F cctagg tatt accaaactca aaga (SEQ ID NO: 48) S9-R GGTCTCaaccaaacaaa gatttggtga atg (SEQ ID NO: 49)

The location of the products obtained by RT-PCR for the La Sota strain gene is shown in FIG. 8.

A diagram of vector inserted 8 parts of the La Sota strain gene into TA-cloning vector, respectively, is shown in FIG. 9A, and the size of fragments inserted in the vector that was treated with EcoRI is shown in FIG. 9A using PCR.

2.2.2. Cloning and Sequence Analysis of NDV Full-Length cDNA

After the amplified PCR product was assessed by electrophoresis on an agarose gel, the obtained gel was purified by GenClean III™ (Qbio Co.), and cloned with a Topo cloning kit (Invitrogen) or an XL-Topo cloning kit (Invitrogen). All nucleotide sequences of each obtained clone were analyzed using primers such as M13 forward, M13 reverse, etc. on the vector, or using primers shown in Table 9. As a result, clones having the same nucleotide sequence as the previous known La Sota strain, namely those in which no mutation occurred, were selected.

TABLE 9 Primers used for analyzing a nucleotide sequence of La Sota strain genome Primer Primer sequence (5′->3′) La-601 taccctggagaggatcctc (SEQ ID NO: 50) La-1261 cgagctaaagctaaccccag (SEQ ID NO: 51) La-1901 agatgcagagatcgacgagc (SEQ ID NO: 52) La-2581 aggcgatatcacagagagta (SEQ ID NO: 53) La-3271 gtgccccaattgtgccaag (SEQ ID NO: 54) S6-F-La actagttgagatcctcaaagatgacgg (SEQ ID NO: 55) S6-R-La tgctctgccctttcaggaccggagctcgccatg (SEQ ID NO: 56) S7-F-La catggcgagctccggtcctgaaagggcagagca (SEQ ID NO: 57) La-5121 cagctcaggaattagactgc (SEQ ID NO: 58) La-5711 gtcatcgccaactgcaagat (SEQ ID NO: 59) La-7042 ctccggacatctgcaacag (SEQ ID NO: 60) La-8591 aaactcggaagggcagtac (SEQ ID NO: 61) La-9311 ttcgcattcaacctgcagg (SEQ ID NO: 62) La-9971 cttagagatgacaatgtggc (SEQ ID NO: 63) La-10661 gtaagatcagacgactctcc (SEQ ID NO: 64) La-11321 tttgagactgttgcaagcc (SEQ ID NO: 65) La-12012 tgtcgccacatgtaaaggc (SEQ ID NO: 66) La-12721 tacccgaaattggatcagtg (SEQ ID NO: 67) La-13339 catgtctcggaagagcctc (SEQ ID NO: 68) La-13981 atctgcagtgccctacaga (SEQ ID NO: 69) La14976 acagtaactgtgactcttaacgaaaatcacatattaataggctcc (SEQ ID NO: 70) La15020R ggagcctattaatatgtgattttcgttaagagtcacagttactgt (SEQ ID NO: 71) S7-R gatccgtacgaatgctgctgaactc (SEQ ID NO: 72) NDV-Pt-R tgccactgmtagttgygata (SEQ ID NO: 73) NDcomF156 atacacctcrtcycagacag (SEQ ID NO: 74) La-8892R gagccatgcaaacttggctgtggacc (SEQ ID NO: 75) La-14708 acagtgcacgagacagatcc (SEQ ID NO: 76) La-15092R gtcctaaggagtcagggttc (SEQ ID NO: 77)

All clones in which no mutation occurred in the nucleotide sequence were sequentially cloned into multi-cloning sites of the parental vector pTMH, respectively, as shown in FIG. 10A and FIG. 10B. Also, a new restriction recognition site was introduced between L genes. The cloning process is shown in FIG. 10A.

2.2.3. Preparation of a Vector for Expression of NP, P and L Proteins Used for Forming RNP Complex

To prepare a vector for expression of NP, P and L proteins of the Newcastle virus, the NP, P, and L genes of the La Sota strain were amplified by RT-PCR reaction using primers shown in Table 10, respectively, and the amplified product was cloned into TA-cloning vector. After sequencing analysis for the clones, only clones in which no mutation occurred in the nucleotide sequence were treated with NotI, and subcloned into the NotI site of the pcDNA6N5 vector.

TABLE 10 Primers used for amplifying and cloning of NP, P, and L genes of La Sota strain Primer Primer sequence (5′->3′) NDV-NP-F gagcggccgc-accatgagtacgagcagctcc (SEQ ID NO: 78) NDV-NP-R gagcggccgc-tcagtacccccagtcggtg (SEQ ID NO: 79) NDV-P-F gagcggccgc-accatggccacctttacagatg (SEQ ID NO: 80) NDV-P-R gagcggccgc-ttagccatttagagcaaggc (SEQ ID NO: 81) NDV-L-F gagcggccgc-accatggcgagcctccgatcctgaaa (SEQ ID NO: 82) NDV-L-R gagcggccgc-ttaagagtcacagttactgtaatatcc (SEQ ID NO: 83)

The amplified products for NP, P, and L genes obtained by RT-PCR are shown in FIG. 11A. Each gene was cloned into a TA-cloning vector, and the clones in which no mutation occurred in the nucleotide sequence were selected by sequencing analysis. The selected clones are shown in FIG. 11B.

2.3. Preparation of a Recombinant Lentogenic NDV with a Surface Antigen of Velogenic Field NDV

2.3.1. Preparation of a Recombinant Clone Attenuating F and HN Gene of Velogenic KBNP-4152

In an embodiment of the present invention, a recombinant vector for transcription of the NDV genome wherein F and HN gene of the velogenic Newcastle disease virus, KBNP-4152 that is recently prevalent domestically was inserted in a vector for transcription of the La Sota strain (rNDV) using the La Sota strain as a backbone, which is manufactured.

2.3.2. Synthesis of a Recombinant Viral Gene

Each of the modified F and HN gene was obtained using site-directed mutagenesis or PTDS (PCR DNA synthesis) (Xiong, A. S. et al., 2004, Nucleic Acids Research, Vol 32, No. 12 e98). The manufacturing process is shown in FIG. 12 to 15.

(A) A gene having a 3′-terminus of the La Sota M gene, a linker of the KBNP-4152 F gene, and a modified nucleotide sequence of the cleavage site of KBNP-4152 F gene were synthesized.

For manufacturing a recombinant virus, two genes having genomic sequences of the La Sota strain up to the 3′-terminus of the M gene, and having nucleotide sequences of KBNP-4152 up to the intergenic sequence and gene start sequence, were fused (refer to FIG. 13). For attenuating the F gene, the gene was mutated so that occurred the codons for the amino acids located at the cleavage site encoded one or two basic amino acids (i.e., changed from 112-RRQKRF-117 into 112-GRQARL-117). As shown in FIG. 14, primers were synthesized, and primers combined according to the arrow direction were added. Then, the gene was artificially synthesized by site-directed mutagenesis and PTDS.

(B) Then, KBNP-4152 HN (1-569) gene and an HN gene terminus (570-577) of the La Sota strain were connected. As a result, nucleotide sequences of the connected sites were as follows (SEQ ID NO: 85, the underlined part is an HN C-terminus coding sequence of the La Sota strain).

ccctttACTAGTTGAGATTCTCAAGGATGATGGGGTTAGGGAGGCCA GGGCTGGCCGCTT GAGTCAATTGCGAGAGGGTTGGAAAGATGACATTGTATCACCTAT CTTTTGCGACGCCAA GAATCAAACTGAGTACCGGCGTGAGCTCGAGTCTTACGCTGCCAG CTGGCCATAATCAGC TAGCGCTAATGTGATTAGATTAAATCTTGTCGATAGTCACTTGATT AAGAAAAAATGTAA GTGGCAATGAGATACAAGGCAAAACAGCTCATGGTAAATAATAC GGGTAGGACATGGCGA

(C) As described above, for connecting the KBNP-4152 HN (1-569) gene and HN gene terminus (570-577) of the La Sota strain, primers were designed as shown in FIG. 15, and PTDS was performed.

2.3.3. Chimera NDV (KBNP-C4152R2L) Rescue

After culturing a HEp-2 (Korea Collection for Type Cultures) or BHK21 (received from Ph. D Park M H of Mogam Biotechnology Research Institute) cell line at 80% confluence in 6-well plates (37° C., 5% CO2), vaccinia T7 virus (received from Ph. D Park M H of Mogam Biotechnology Research Institute) was infected. For forming an RNP complex of NDV on the cell line, the three expression plasmid, pcDNA3.1-NP, pcDNA3.1-P and pcDNA3.1-L vector (FIG. 11B) for expression of NP, P and L genomic genes, and the one transcription vector of NDV genome, pTMH C4152-R2L (SEQ ID NO: 1, refer to FIG. 23), were prepared. To manufacturing a recombinant vector, pTMH C4152-R2L, attenuated F gene and the HN (1-569) gene of KBNP-4152 were inserted between the intergenic sequence site at the back of M gene and the 570th amino acid encoding site in the HN gene of the La Sota strain genome.

10 μ

of Lipofectamine™ (Invitrogen Co.) was mixed with 4 μg of plasmid DNA mixed with each plasmid vector at the ratio of 1:1:0.1:1, and transfected to HEp-2 cell line. Then, 1 μ

/ml of acetylated trypsin was added to the culture medium. The cell line was cultured in 6-well plates at 37° C. for 2-3 days, and the cultured cells and culture medium were harvested and performed 3 times of quick freezing and quick defrost. Then, the cells and the culture medium were inoculated into SPF embryonated eggs at 9-11 days old, and allantoic fluid of the embryonated egg was harvested. As a result, a Newcastle disease virus from the allantoic fluid was obtained, and the virus was named a KBNP-C41152R2L strain. The KBNP-C41152R2L strain was deposited with the Korean Collection for Type Culture (Korea Research Institute of Bioscience and Biotechnology, Taejon, Republic of Korea) on Sep. 12, 2006, and assigned deposition No. KCTC10984BP.

The process of manufacturing the KBNP-C4152R2L virus is shown in FIG. 16.

According to the same method as described above, velogenic NDV (named RRQKRF; KBNP-4152R4L) having 4 of basic amino acids and lentogenic NDV (name GGQARL; KBNP-C4152R1L) having 1 of basic amino acid at a cleavage site of the F protein were manufactured, respectively. The name of the strain was determined according to the number of basic amino acids of a cleavage site of the F protein, wherein the number at the back of R indicates the number of basic amino acids among 112 to 116 amino acids, and L (long) indicates the length of HN protein having 577 amino acids similar to the La Sota strain.

Achematic diagram of the obtained KBNP-C4152R1L, KBNP-C4152R2L and KBNP-C4152R4L is shown in FIG. 17.

Example 3 Analysis of a KBNP-C4152R2L

3.1. General Characteristics Analysis of Chimera NDV

The obtained KBNP-C4152R2L was inoculated into SPF embryonated eggs at 9-10 days old, and allantoic fluid of the embryonated egg was harvested after 3-5 days. Then, it was confirmed whether the recombinant NDV grows or not by using a plate hemagglutination test. The results are shown in FIG. 18. As shown in FIG. 18, hemagglutination was observed in the allantoic fluid obtained from the embryonated egg inoculated with KBNP-C4152R2L, and consequently the growth of KBNP-C4152R2L was confirmed.

KBNP-C4152R2L is the same as the KBNP-4152 wherein most of the F and HN genes are field velogenic, but it is a virus wherein the cleavage site of the F protein is artificially attenuated. Particularly, the viral RNA was not detected by PCR using a velogenic NDV-specific primer, but was detected by PCR using all NDV common detecting primers. This fact was confirmed by a gene marker of the recombinant NDV through determining the amplified viral RNA sequences obtained by RT-PCR using NDV-pt-R and NDVcomF156 primers shown in Table 5. By the RT-PCR method, the chimera virus was detected, and the pathogenic type (pathotype) was confirmed. The results are shown in FIG. 19. As shown in FIG. 19, KBNP-C4152R2L is detected by PCR using NDV commonly primer (NDVcomF156), but is not detected by PCR using a pathogenic strain-specific primer (NDV-pt-R) (Korea Patent No. 0451883).

For identifying the virus in more detail, the nucleotide sequence of the virus was analyzed after RT-PCR, and the nucleotide sequence coding the cleavage site of the F gene which is artificially synthesized was confirmed. The results are shown in FIG. 20. As shown in FIG. 20, the cleavage site of the F protein of parental strain, KBNP-4152 has a structure of 112-RRQKRF-117, but KBNP-C4152R2L has a structure of 112-GRQARL-117. Particularly, NDV in nature does not have the alanine (A) at the 115^(th) amino acid position, but the chimer virus of the present invention has the alanine. This is a feature of the present invention of only the chimera virus.

3.2. Serologic Characteristics Analysis of Chimera NDV

Because F and HN of KBNP-C4152R2L are similar to KBNP-4152 but are clearly different from the La Sota strain, it is expected that KBNP-C4152R2L is serologically similar to KBNP-4152 but different from the La Sota strain. In order to confirm this, serologic characteristics were analyzed by using a cross hemagglutination inhibition test. The results are shown in FIG. 21. As shown in FIG. 21, serologically KBNP-C4152R2L is similar with KBNP-4152. That is, when compared with the La Sota strain, anti-serum of anti-La Sota showed 4 to 8 times lower hemagglutination units in the KBNP-C4152R2L and KBNP-4152, and anti-serum of anti-KBNP-4152 showed nearly quite similar hemagglutination units in the oneself or KBNP-C4152R2L.

3.3. Pathogenicity Index Measurement of a KBNP-C4152R2L

3.3.1. Mean Death Time (MDT) Measurement

MDT measurement was performed according to the method of Alexander (1998). The KBNP-C4152R2L virus diluted with 10⁻⁶ was inoculated into two groups of 5 embryonated eggs per group. The first group was inoculated at 9 A.M., and the second group was inoculated at 5 P.M. After inoculation, the eggs were incubated at 37° C. for 7 days, and checked embryo-death-time at 9 A.M. and 5 P.M. each day. Then, mortality time was recorded and MDT (mean death time) was calculated. If the measured MDT was shorter than 60 h, the virus was determined as velogenic, if the measured MDT is 60-90 h, it was determined as mesogenic, and if the measured MDT is 90-120 h, it was determined as lentogenic, and if the measured MDT is more than 120 hours, it was determined apathogenic. MDT of a vaccine virus must be more than 90 h. Consequently, the average mortality time of the KBNP-C4152R2L was measured at more than 168 h, so the KBNP-C4152R2L was classified as apathogenic NDV.

3.3.2. Intracerebral Pathogenicity Index (ICPI) Measurement

After inoculating 50 μ

of allantoic fluid (Example 2.3.3.) diluted with sterile saline 10-fold into intracerebrally into 1 day-old chicks (n=10) using a 1 cc syringe, a normal chick is marked as 0, a chick showing a disease symptom was marked as 1, and a killed chick was marked as 2, respectively. Then, the overall score of each group obtained over 8 days was divided by 80. In the results, apathogenic was classified as 0.0-0.2, lentogenic was classified as 0.2-0.5, mesogenic was classified as 1.0-1.5, and velogenic was classified as 1.5-2.0. For utilizing the virus as a vaccine virus domestically, because the pathogenicity index must be less than 0.5, a recombinant virus having 0.0-0.5 of pathogenicity index was selected.

The results of measurements of KBNP-4152, KBNP-C4152R2L and ICPI of the La Sota strain are shown in Tables 11 to 13, respectively.

TABLE 11 ICPI measurement of KBNP-4152 Day 1 2 3 4 5 6 7 8 Sum(index) Sign 6 0 0 0 0 0 0 0  6 × 1 Dead 4 10 10 10 10 10 10 10 74 × 2 Normal 0 0 0 0 0 0 0 0 Total = 154 ICPI = 154/80 = 1.925

TABLE 12 ICPI measurement of KBNP-C4152R2L Day 1 2 3 4 5 6 7 8 Sum(index) Sign 0 0 0 0 0 0 0 0 0 × 1 Dead 0 0 0 0 0 0 0 0 0 × 2 Normal 10 10 10 10 10 10 10 10 Total = 154 ICPI = 0/80 = 0.0

TABLE 13 ICPI measurement of La Sota strain Day 1 2 3 4 5 6 7 8 Sum(index) Sign 0 0 0 0 0 3 5 2 10 × 1 Dead 0 0 0 0 0 0 1 4  5 × 2 Normal 10 10 10 10 10 10 10 10 Total = 20 ICPI = 20/80 = 0.25

According to the MDT and ICPI results, MDT of the KBNP-C4152R2L was more than 168 h, and ICPI of the KBNP-C4152R2L was 0.0. Therefore, the virus was confirmed as a virus without any pathogenicity. This result of the pathogenicity decrease indicates that the KBNP-C4152R2L strain of the present invention is attenuated more than the conventional vaccine strain, La Sota, and therefore the pathogenicity of the KBNP-C4152R2L strain is less than that of the La Sota strain. A sudden decrease of the pathogenicity in the cytopathic effect is capable of being shown in the recombinant virus introduced with the F and HN genes of velogenic field NDV strain having a granular cytopathic effect instead of the F and HN genes of the strain having an excellent syncytial formation capability. Thus far, it has been reported that a clone having a syncytial type and granular type of cytopathic effect among velogenic NDV exists. Further, it has been reported that the ICPI value (1.78) of the granular type of clone, SNU9358GG (AF535861) is less than the ICPI value (1.95) of syncytial type of NDV clone, SNU9358GS (received from Avian Disease & Laboratory, College of Veterinary Medicine of Seoul National University), and therefore the pathogenicity of the granular type of clone is less than that of the syncytial type of NDV clone. However, because the NDV clone having this granular type of cytopathic effect in the pathogenicity NDV with other genotypes III, IV, V, VIII, and XI including genotype VII was not previously known, the present inventors succeeded for the first time in cloning a granular type of NDV from the NDV with genotype VII, and manufacturing a recombinant NDV having pathogenicity of less than the La Sota strain having a granular type of cytopathic effect using F and HN genes of the virus.

3.4. Genetic Safety Test of a KBNP-C4152R2L

After sub-culturing a recombinant virus of the present invention, KBNP-C4152R2L on a chicken embryo more than 9 times, 4030-8889 nucleotide sequence of the chimera genome was amplified by RT-PCR and analyzed by DNA sequencing. As a result, the nucleotide sequence had no change on the nucleotide sequence, and it was confirmed as a very stable nucleotide sequence.

3.5. Productivity Measurement of a KBNP-C4152R2L: EID50 (50% Egg-Infectious Dose) Measurement

After inoculating 0.1 ml of the virus diluted 10-fold with a sterile saline solution into the allantoic cavity of 5-7 chicken embryos at 9-10 days old, the inoculated chicken embryos were cultivated at 37° C. for 5-7 days. Then, the obtained chicken embryos were chilled, and the allantoic fluid from the chilled chicken embryos were harvested. Then, it was determined whether the virus increased or not by using hemagglutination, which was calculated according to a determined formula. As a result, EID50 of KBNP-C4152R2L was observed at 1010.1/m

, and the productivity of the virus was very high, similar to the previous La Sota strain.

3.6. Hemagglutination-Elution Pattern Measurement

The examination for hemagglutination-elution was performed according to the method of Spalatin (1970). Namely, 0.5 ml of each of the La Sota strain and KBNP-C4152R2L serially diluted with CMF-PBS in 2-fold and 0.25 ml of 1% chicken blood were mixed in micro-well plates. After incubating the plates at room temperature for 1 h, the hemagglutination titer was measured. After the 1^(st) reading, the plate was maintained at 24° C. for 24 h, and the hemagglutination titer was measured again. The plate was resuspended after 2 h, and the hemagglutination titer again was again measured. The titer was represented as the reciprocal of the hemagglutination-occurring maximal dilution factor. A method of determining the hemagglutination-elution pattern is as follows, when the hemagglutination-elution had completely occurred after hemagglutination at 24 h, and the hemagglutination had not occurred after resuspension at 2 h, the hemagglutination-elution pattern was determined as a rapid eluter, but determined as a slow eluter.

As a result, the La Sota strain was observed as a slow eluter, but the KBNP-C4152R2L was observed as a rapid eluter, indicating that the KBNP-C4152R2L has the same biological characteristics as the KBNP-4152.

3.7. Cytopathic Effect Measurement

After culturing chicken embryonic fibroblast (CEF) to form a single layer on 96-well plates, the La Sota strain, KBNP-4152 and KBNP-C4152R2L (each 200 TCID50/well) were infected into the cultured cells, respectively. Then, the culture medium was replaced with a medium containing 0.5% FBS and 20 μg/m

of trypsin. Observed results after culturing for 3 days, are shown in FIG. 22. The red arrow in the middle of FIG. 22 indicates the formation of syncytia of typical cytopathic effects of NDV. Practically, it was confirmed that KBNP-C4152R2L forms a specific syncytia of NDV when trypsin is added in the strain (the red arrow of FIG. 22).

This cytopathic effect indicates that the chimera virus is a lentogenic pathogenicity strain having trypsin dependency in the formation of syncytia. Further, the virus has a granular type of cytopathic effect as shown in FIG. 22D and without the formation of typical syncytia, different from the La Sota strain and the KBNP-4152 strain.

3.8. Killed Vaccine Test of a KBNP-C4152R2L

A chicken embryo culture medium of KBNP-C4152R2L, a 10-fold concentrated fluid obtained from the culture medium, and a chicken embryo culture medium of the La Sota strain were each inactivated by adding 0.3% formaldehyde. For manufacturing each of the killed vaccines, 30% of each of the inactivated viral antigen supernatants was mixed with 70% ISA70 oil. The manufactured killed vaccine was inoculated into the subcutaneous tissue of 6-week-old SPF chickens. After 3 weeks, a correction circumference 106TCID50 (AY6304009) used as a standard in Quarantine service was challenged into the nasal cavity and oral cavities. After the challenge, the mortality rate up to 10 days was observed. The results are shown in Table 14.

TABLE 14 Protection efficacy and immunogenicity of Chimera NDV vaccine Vaccine Dose HI titer Survival After (killed oil) HA unit EID₅₀ Test antigen 6 DPV 21 DPV challenge Control — — La Sota 0 0  1/10 C4152R2L 0.2 ± 0.6 0  (10%) KBNP-4152 0 0 C4152R2L 20 10^(9.3) La Sota 0.3 ± 0.8 7.7 ± 1.3 15/15 oil C4152R2L 0.2 ± 0.8 8.2 ± 0.9 (100%) KBNP-4152 0.2 ± 0.8 7.7 ± 1.0 C4152R2L 154 10^(9.9) La Sota 1.5 ± 1.4 7.7 ± 1.2 15/15 oil C4152R2L 2.2 ± 1.4 8.9 ± 1.3 (100%) KBNP-4152 2.2 ± 1.4 8.2 ± 1.3 BNE oil 154 10^(9.5) La Sota 0.2 ± 0.6 6.9 ± 1.5 8/9 C4152R2L 0 5.1 ± 1.4  (89%) KBNP-4152 0 4.6 ± 1.5

As shown in Table 14, even though the antigen amount of KBNP-C4152R2L calculated by HA titer is lower compared to the La Sota strain, the antibody formation capability of KBNP-C4152R2L is much better compared with previous vaccines. Particularly, the serological immunity against the field strain was better with the KBNP-C4152R2L compared to the La Sota strain.

3.9. In Ovo Vaccine Test of a KBNP-C4152R2L

KBNP-C4152R2L has lower pathogenicity than a strain used for the current vaccine, and its possibility for in ovo vaccine is high. For confirming this, in ovo vaccine was inoculated into 18 day-old chicken embryos, and the hatching rate and the rate of weight gain obtained after hatching at 2 weeks were compared with a control group. By examining the antibody titer of the inoculated chicken embryos at 2 weeks old or more, it was determined whether maternal antibodies were overcome or not, and the immunity levels were compared. After 2 weeks, the velogenic virus was challenged into the chicken embryos, and the rate of protection against the virus was determined.

Chicken embryo used for the test was conventional laying hen chicks, and 0.1 cc of each diluted vaccine strain (10^(7.0) EID₅₀/ml) was inoculated into the chicken embryos at 18 days of age. A negative control group was inoculated with 0.1 cc of sterile PBS. To confirm the level of maternal antibodies, 5 chicken embryos of a negative control group were euthanized immediately after hatching and the serum of the euthanized chicken embryos was obtained. After 17 days of age, individual weights for each group were measured and blood was drawn. Then, a virulent stain KBNP-4152 (10^(6.5) EID₅₀) was inoculated into nasal cavities and oral cavities thereof. After the attack inoculation, the survival rate to 10 days was observed. The results are shown in Table 15.

TABLE 15 Comparison of in ovo vaccine effect of a KBNP-C4152R2L Hatchability and protection of chickens inoculated with chimera NDV, strain, KBNP-C4152R2L at 18 day of embryonation against velogenic NDV, challenge. Survival from Mean Dose hatching Weight Mean HI titer (log_(e)) Survival Vaccine log₁₀ to 17 at 17 Test at 1 at 17 After (live) EID₅₀/egg day-old day-old antigen day old day-old Challenge Control — 23/27 (85.2%) 201 ± 17.8 g La Sota 5.8 ± 2.7 2.7 ± 1.7 12/18 C4152R2L — 1.8 ± 1.3 (66.7%)   C4152R2L 6.1 17/20 (85.0%)   204 ± 42 g La Sota — 4.9 ± 1.1 17/17 C4152R2L — 5.5 ± 1.4 (100%) La Sota 6.0  4/20 (20.0%) — La Sota — 6.8 ± 2.2 4/4 C4152R2L — 5.0 ± 1.4 (100%)

As shown in Table 15, the KBNP-C4152R2L inoculation group and the negative control group had no statically significant difference in hatching rate and rate of weight gain. However, the antibody titer at 17 days after hatching was higher in the KBNP-C4152R2L inoculation group compared with the control group. That is, in the case of the control group, the hemagglutination inhibition (HI) titer of the maternal antibody at 1 day versus the La Sota strain was decreased by 2.7±1.7 from an average of 5.8±2.7. On the other hand, in the case of the KBNP-C4152R2L inoculation group, the HI titer of maternal antibody at 1 day versus the La Sota strain was 4.9±1.1, and showed a relatively higher HI titer of 5.5±1.4. Further, after challenge, a mortality rate of 33% was observed in the control group, but a survival rate of 100% was observed in the recombinant vaccine inoculation group.

These results are excellent compared to “U.S. Pat. No. 6,699,479B1” of the title “Recombinant Newcastle disease virus as an embryo vaccine” by Akzo Nobel N/6 V (NL) Company, and is significant in that the vaccine is obtained from KBNP-C4152R2L developed by a new method without using attenuation according to P gene editing.

Recently, developed countries have preferred in ovo vaccines that are capable of directly vaccinating chicken embryos before hatching, because these vaccines have the simplicity of inoculation and economy. However, there has been a limit in live vaccine strains known thus far that are applied to Newcastle disease, because the strains have pathogenicity with respect to chicken embryos. On the other hand, it is confirmed that KBNP-C4152R2L of the present Invention has no pathogenicity in the chicken embryos, and it is expected that the value of applying the KBNP-C4152R2L as in ovo vaccine is very high.

As described above, the Newcastle disease virus of the present invention has similar antigenicity to the velogenic Newcastle disease virus that is prevalent both domestically and in Asia as a whole. Further, because the Newcastle disease virus of the present invention has similar or significantly low pathogenicity compared with currently used vaccine strains, the vaccine can be used as in ovo vaccine. In addition, the possibility of acquisition of pathogenicity by point mutation is significantly lower compared with previous vaccine strains. Therefore, the Newcastle disease virus of the present invention can be used in manufacturing killed vaccine, a live vaccine and in ovo vaccine for preventing Newcastle disease in both domestically and in Asia overall. 

1. A strain of Newcastle disease virus, comprising nucleotide sequences encoding NP, P, M, and L proteins of a low-pathogenic Newcastle disease virus AY845400; and nucleotide sequences encoding F and HN proteins of a high-pathogenic Newcastle disease virus KCTC 10919BP, wherein the F protein coding sequence comprised in the strain is characterized by substitution of the codon encoding the 115^(th) amino acid of the F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU.
 2. A strain of Newcastle disease virus, comprising nucleotide sequences encoding NP, P, M, and L proteins of a low-pathogenic Newcastle disease virus AY845400; and nucleotide sequences encoding F and HN proteins of a high-pathogenic Newcastle disease virus KCTC 10919BP, wherein the F protein coding sequence comprised in the strain is characterized by substitution of the codon encoding the 115^(th) amino acid of the F protein of the high-pathogenic Newcastle disease virus with any one selected from the group consisting of alanine codons consisting of GCA, GCC, GCG and GCU; aspartic acid codons consisting of GAC and GAU; phenylalanine codons consisting of UUC and UUU; isoleucine codons consisting of AUC and AUU; leucine codons consisting of UUA and UUG; serine codons consisting of UCA, UCC, UCG and UCU; threonine codons consisting of ACC and ACU; valine codons consisting of GUA, GUC, GUG and GUU; and tyrosine codons consisting of UAC and UAU, wherein the HN protein is a recombinant HN protein having the amino acid sequences of 570^(th) and higher positions from the low-pathogenic Newcastle disease virus and the amino acid sequences from 1^(st) to 569^(th) positions from the high-pathogenic Newcastle disease virus.
 3. The strain according to claim 1, wherein the strain is KCTC 10984BP.
 4. A vaccine against Newcastle disease comprising the stain of Newcastle disease virus of claim
 1. 5. A vaccine against Newcastle disease comprising the stain of Newcastle disease virus of claim
 2. 6. The vaccine according to claim 4, wherein the strain is KCTC 10984BP.
 7. The vaccine according to claim 4, wherein the vaccine is an inactivated killed vaccine or a live vaccine.
 8. A method of delivering the vaccine of claim 4 to a subject, comprising delivering the vaccine to the subject in ovo.
 9. The vaccine according to claim 5, wherein the vaccine is an inactivated killed vaccine or a live vaccine.
 10. A method of delivering the vaccine of claim 5 to a subject, comprising delivering the vaccine to the subject in ovo. 